ArticleMolecular systems biology2006
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.
Article in Molecular systems biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4,631 papers.
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4,631 citing papers in PubMed, 8,134 citations in OpenAlex.
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
We have systematically made a set of precisely defined, single-gene deletions of all nonessential genes in Escherichia coli K-12. Open-reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a one-step method for inactivation of chromosomal genes and primers designed to create in-frame deletions upon excision of the resistance cassette. Of 4288 genes targeted, mutants were obtained for 3985. To alleviate problems encountered in high-throughput studies, two independent mutants were saved for every deleted gene. These mutants-the 'Keio collection'-provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome-wide testing of mutational effects in a common strain background, E. coli K-12 BW25113. We were unable to disrupt 303 genes, including 37 of unknown function, which are candidates for essential genes. Distribution is being handled via GenoBase (http://ecoli.aist-nara.ac.jp/).
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