ArticleRNA (New York, N.Y.)2006
A subset of Mer1p-dependent introns requires Bud13p for splicing activation and nuclear retention.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
- An Arabidopsis Retention and Splicing complex regulates root and embryo development through pre-mRNA splicing.Plant physiology · 2022Article
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Mutagenesis of Snu114 domain IV identifies a developmental role in meiotic splicing.RNA biology · 2019Article
- Identification of factors that promote biogenesis of tRNARNA biology · 2018Article
- Non-vesicular Lipid Transport Machinery inFrontiers in cellular and infection microbiology · 2018Review
- The pre-mRNA retention and splicing complex controls expression of the Mediator subunit Med20.RNA biology · 2017Article
- Nineteen complex-related factor Prp45 is required for the early stages of cotranscriptional spliceosome assembly.RNA (New York, N.Y.) · 2017Article
- Structures of intermediates during RES complex assembly.Scientific reports · 2015Article
- The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing.RNA biology · 2015Article
- A novel protein-protein interaction in the RES (REtention and Splicing) complex.The Journal of biological chemistry · 2014Article
- Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.Nature structural & molecular biology · 2014Article
- The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression.Nucleic acids research · 2013Article
- Article
- Functional consequences of developmentally regulated alternative splicing.Nature reviews. Genetics · 2011Review
- An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Determinants of Nam8-dependent splicing of meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Factors affecting splicing strength of yeast genes.Comparative and functional genomics · 2011Article
- Frame-disrupting mutations elicit pre-mRNA accumulation independently of frame disruption.Nucleic acids research · 2010Article
- Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations.RNA (New York, N.Y.) · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the yeast Saccharomyces cerevisiae, Mer1p is expressed only during meiosis, and its expression is linked to the splicing of at least three mRNAs: MER2, MER3, and AMA1. Previous evidence suggests that Mer1p activates splicing by directly recruiting snRNPs or stabilizing intermediate splicing complexes formed on pre-mRNA that contains an intronic Mer1p enhancer element. However, some splicing factors, especially accessory/non-snRNP factors, have critical roles in retaining unspliced pre-mRNAs in the nucleus. We tested if Mer1p may indirectly regulate splicing by preventing the export of pre-mRNAs to the cytoplasm and also demonstrated that a second subunit of the Retention and Splicing (RES) complex, Bud13p, has transcript-specific effects on Mer1p-activated splicing. The results indicated that Mer1p can retain unspliced pre-mRNA in the nucleus; however, nuclear retention could not be uncoupled from splicing activation. In the absence of Mer1p, the AMA1 pre-mRNA is exported to the cytoplasm, translated, but not subjected to nonsense-mediated decay (NMD) despite a premature stop codon in the intron. These data imply that Mer1p can retain pre-mRNAs in the nucleus only by facilitating their interaction with the spliceosome and that two subunits of the RES complex modulate Mer1p function on two of the three Mer1p-dependent introns. The results also support models for cytoplasmic degradation of unspliced pre-mRNAs that fail to assemble into spliceosomes in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.