ArticleSurgical endoscopy2006
Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1.
Article in Surgical endoscopy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 8 citations in OpenAlex.
- Humidification during laparoscopic surgery: overview of the clinical benefits of using humidified gas during laparoscopic surgery.Archives of gynecology and obstetrics · 2015Review
- Post-transcriptional regulation of plasminogen activator inhibitor type-1 expression in human pleural mesothelial cells.American journal of respiratory cell and molecular biology · 2010Article
- Effect of Reteplase and PAI-1 antibodies on postoperative adhesion formation in a laparoscopic mouse model.Surgical endoscopy · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAbdominal mesothelial cells are exposed to carbon dioxide during laparoscopy. Previous data indicate that carbon dioxide increases release and expression of plasminogen activator inhibitor type-1 (PAI-1) and induces acidification.
methodsTo assess the impact resulting from a range of pH, human mesothelial cells were exposed to culturing media balanced to pH levels of 6.0 to 8.0 for 90 min. Samples from cell media were withdrawn at several time points. Concentrations of PAI-1 and PAI-1 activity were measured using enzyme-linked immunoassay techniques. To focus on the effect of clinically relevant pH, cells were subjected to pH 6.4 and 7.4. Samples were withdrawn for PAI-1 assessments and for PAI-1 mRNA analyses.
resultsDuring exposure to various levels of pH, PAI-1 secretion and activity were variable. However, 5 h after exposure, greater concentration and activity of PAI-1 were observed in acidified cultures. More PAI-1 mRNA was isolated after exposure of cells to a pH of 6.4, apparently indicating transcriptional regulation.
conclusionsMesothelial cells seem to respond to acidification by an increased release and production of PAI-1 in vitro.
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Registered trials
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