ArticleThe Journal of biological chemistry2006
Role of DNA methyltransferases in regulation of human ribosomal RNA gene transcription.
Article in The Journal of biological chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Specific or not specific recruitment of DNMTs for DNA methylation, an epigenetic dilemma.Clinical epigenetics · 2018Review
- Methylation of the ribosomal RNA gene promoter is associated with aging and age-related decline.Aging cell · 2017Article
- The Epigenetic Pathways to Ribosomal DNA Silencing.Microbiology and molecular biology reviews : MMBR · 2016Review
- Morphology of nuclear transcription.Histochemistry and cell biology · 2016Review
- Overexpression of Ribosomal RNA in the Development of Human Cervical Cancer Is Associated with rDNA Promoter Hypomethylation.PloS one · 2016Article
- Loss of nucleolar histone chaperone NPM1 triggers rearrangement of heterochromatin and synergizes with a deficiency in DNA methyltransferase DNMT3A to drive ribosomal DNA transcription.The Journal of biological chemistry · 2014Article
- The nucleolar size is associated to the methylation status of ribosomal DNA in breast carcinomas.BMC cancer · 2014Article
- Epigenetics of eu- and heterochromatin in inverted and conventional nuclei from mouse retina.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- Neuronal pentraxin II (NPTX2) is frequently down-regulated by promoter hypermethylation in pancreatic cancers.Digestive diseases and sciences · 2012Article
- Molecular and organismal changes in offspring of male mice treated with chemical stressors.Environmental and molecular mutagenesis · 2012Article
- Reduced susceptibility of DNA methyltransferase 1 hypomorphic (Dnmt1N/+) mice to hepatic steatosis upon feeding liquid alcohol diet.PloS one · 2012Article
- siDNMT1 increases γ-globin expression in chemical inducer of dimerization (CID)-dependent mouse βYAC bone marrow cells and in baboon erythroid progenitor cell cultures.Experimental hematology · 2011Article
- Mechanisms of epigenetic silencing of the Rassf1a gene during estrogen-induced breast carcinogenesis in ACI rats.Carcinogenesis · 2010Article
- Methylation of histone H3 and H4 by PRMT5 regulates ribosomal RNA gene transcription.Journal of cellular biochemistry · 2010Article
- Potential of DNMT and its Epigenetic Regulation for Lung Cancer Therapy.Current genomics · 2009Article
- DNA Methyltransferase protein synthesis is reduced in CXXC finger protein 1-deficient embryonic stem cells.DNA and cell biology · 2009Article
- Association of differential and site-dependent CpG methylation and diverse expression of DNA methyltransferases with the tissue-specific expression of human beta-globin gene in transgenic mice.International journal of hematology · 2009Article
- Chromatin: linking structure and function in the nucleolus.Chromosoma · 2009Review
- DNA methyltransferase 3B mutant in ICF syndrome interacts non-covalently with SUMO-1.Journal of molecular medicine (Berlin, Germany) · 2008Article
- Structure and function of ribosomal RNA gene chromatin.Biochemical Society transactions · 2008Review
Corrections and comments
- Retraction · 2018-02-13Concerns/Issues about Image · Objections by Author(s) · Original Data and/or Images not Provided and/or not Available · Unreliable Image ·
- Retracted
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We have previously demonstrated that the expression of human ribosomal RNA genes (rDNA) in normal and cancer cells is differentially regulated by methylation of the promoter CpG islands. Furthermore, we showed that the methyl CpG-binding protein MBD2 plays a selective role in the methylation-mediated block in rDNA expression. Here, we analyzed the role of three functional mammalian DNA methyltransferases (DNMTs) in regulating the rDNA promoters activity. Immunofluorescence analysis and biochemical fractionation showed that all three DNMTs (DNMT1, DNMT3A, and DNMT3B) are associated with the inactive rDNA in the nucleolus. Although DNMTs associate with both methylated and unmethylated rDNA promoters, DNMT1 preferentially associates with the methylated genes. The rDNA primary transcript level was significantly elevated in DNMT1-/- or DNMT3B-/- human colon carcinoma (HCT116) cells. Southern blot analysis demonstrated a moderate level of rDNA promoter hypomethylation in DNMT1-/- cells and a dramatic loss of rDNA promoter methylation in double knockout cells. Transient overexpression of DNMT1 or DNMT3B suppressed the luciferase expression from both methylated and unmethylated pHrD-IRES-Luc, a reporter plasmid where the rDNA promoter drives luciferase expression. DNMT1-mediated suppression of the unmethylated promoter involves de novo methylation of the promoter, whereas histone deacetylase 2 cooperates with DNMT1 to inhibit the methylated rDNA promoter. Unlike DNMT1, both the wild type and catalytically inactive DNMT3B mutant can suppress rDNA promoter irrespective of its methylation status. DNMT3B-mediated suppression of the rDNA promoter also involves histone deacetylation. Treatment of HCT116 cells with Decitabine (a DNMT inhibitor) or trichostatin A (a histone deacetylase inhibitor) up-regulated endogenous rDNA expression. These inhibitors synergistically activated methylated pHrD-IRES-Luc, whereas they exhibited additive effects on the unmethylated promoter. These results demonstrate localization of DNMTs with the inactive rDNA in the nucleolus, the specific role of DNMT1 and DNMT3B in rDNA expression and the differential regulation of rDNA expression from the methylated and unmethylated rDNA promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.