ArticleProceedings of the National Academy of Sciences of the United States of America2006
Bending a membrane: how clathrin affects budding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 124 citations in OpenAlex.
- Article
- Induced nanoscale membrane curvature bypasses the essential endocytic function of clathrin.The Journal of cell biology · 2022Article
- Find your coat: Using correlative light and electron microscopy to study intracellular protein coats.Current opinion in cell biology · 2021Review
- Dynamic instability of clathrin assembly provides proofreading control for endocytosis.The Journal of cell biology · 2019Article
- The Molecular Architecture of Native BBSome Obtained by an Integrated Structural Approach.Structure (London, England : 1993) · 2019Article
- Entry modes of ellipsoidal nanoparticles on a membrane during clathrin-mediated endocytosis.Soft matter · 2019Article
- Stochastic simulations of nanoparticle internalization through transferrin receptor dependent clathrin-mediated endocytosis.Biochimica et biophysica acta. General subjects · 2018Article
- The molecular machines that traffic signaling receptors into and out of cilia.Current opinion in cell biology · 2018Review
- Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics.Nature communications · 2018Article
- Role of clathrin in dense core vesicle biogenesis.Molecular biology of the cell · 2017Article
- Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission.Nature communications · 2017Article
- Transient Fcho1/2⋅Eps15/R⋅AP-2 Nanoclusters Prime the AP-2 Clathrin Adaptor for Cargo Binding.Developmental cell · 2016Article
- Durable protein lattices of clathrin that can be functionalized with nanoparticles and active biomolecules.Nature nanotechnology · 2015Article
- A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats.Nature communications · 2015Article
- Flat clathrin lattices: stable features of the plasma membrane.Molecular biology of the cell · 2014Article
- Endocytic accessory factors and regulation of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Multiscale computational models in physical systems biology of intracellular trafficking.IET systems biology · 2014Article
- Membrane fluctuations destabilize clathrin protein lattice order.Biophysical journal · 2014Article
- Zooming in on the molecular mechanisms of endocytic budding by time-resolved electron microscopy.Cellular and molecular life sciences : CMLS · 2014Review
- Clathrin-coated vesicles from brain have small payloads: a cryo-electron tomographic study.Journal of structural biology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Receptor-mediated endocytosis of ligands, such as transferrin and LDL, is suppressed when clathrin synthesis is blocked by RNA interference in HeLa cells. We have found that domains containing the adapter complex 2 (AP2)-coated vesicle adapter and the endocytic accessory proteins CALM (clathrin assembly lymphoid myeloid leukemia protein), epsin, and eps15/eps15R (EGF receptor pathway substrate 15-related) nevertheless persist at the plasma membrane. They are similar in size and number to those seen in clathrin-expressing cells. Here we characterize these membrane domains by fluorescence and electron microscopy in detail. Fluorescence recovery after photobleaching measurements suggest that the exchange between membrane-bound and free cytosolic AP2 molecules is not significantly influenced by the depletion of clathrin. The AP2 membrane domains are dispersed upon interfering with protein-protein interactions that involve the alpha appendage domain of AP2. Electron microscopy of cellular cortices revealed that the AP2 membrane domains lack any curvature, suggesting that clathrin is essential for driving coated pit invagination. A model for coated vesicle formation, incorporating a mechanism commonly referred to as a "Brownian ratchet," is consistent with our observations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.