ArticleBiochemistry2006
Biphasic Ca2+-dependent switching in a calmodulin-IQ domain complex.
Article in Biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- NaStructure (London, England : 1993) · 2021Article
- Single-Channel Resolution of the Interaction between C-Terminal CaBiophysical journal · 2019Article
- Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP.Nature communications · 2018Article
- Direct visualization of interaction between calmodulin and connexin45.The Biochemical journal · 2017Article
- Calcium triggers reversal of calmodulin on nested anti-parallel sites in the IQ motif of the neuronal voltage-dependent sodium channel NaBiophysical chemistry · 2017Article
- Article
- Preassociated apocalmodulin mediates Ca2+-dependent sensitization of activation and inactivation of TMEM16A/16B Ca2+-gated Cl- channels.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Calcineurin determines toxic versus beneficial responses to α-synuclein.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Visualizing CaMKII and CaM activity: a paradigm of compartmentalized signaling.Journal of molecular medicine (Berlin, Germany) · 2013Review
- NMR studies of the interaction of calmodulin with IQ motif peptides.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Calmodulin binding proteins provide domains of local Ca2+ signaling in cardiac myocytes.Journal of molecular and cellular cardiology · 2012Review
- Insights into modulation of calcium signaling by magnesium in calmodulin, troponin C and related EF-hand proteins.Biochimica et biophysica acta · 2011Review
- Allosteric effects of the antipsychotic drug trifluoperazine on the energetics of calcium binding by calmodulin.Proteins · 2010Article
- Article
- Variations at the semiconserved glycine in the IQ domain consensus sequence have a major impact on Ca2+-dependent switching in calmodulin-IQ domain complexes.Biochemistry · 2010Article
- Article
- Dissecting cooperative calmodulin binding to CaM kinase II: a detailed stochastic model.Journal of computational neuroscience · 2009Article
- Calmodulin mediates differential sensitivity of CaMKII and calcineurin to local Ca2+ in cardiac myocytes.Biophysical journal · 2008Article
- Article
- Crystal structure of the CaV2 IQ domain in complex with Ca2+/calmodulin: high-resolution mechanistic implications for channel regulation by Ca2+.Structure (London, England : 1993) · 2008Article
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3 authors.
Funding
Abstract
The relationship between the free Ca2+ concentration and the apparent dissociation constant for the complex between calmodulin (CaM) and the neuromodulin IQ domain consists of two phases. In the first phase, Ca2+ bound to the C-ter EF hand pair in CaM increases the Kd for the complex from the Ca2+-free value of 2.3 +/- 0.1 microM to a value of 14.4 +/- 1.3 microM. In the second phase, Ca2+ bound to the N-ter EF hand pair reduces the Kd for the complex to a value of 2.5 +/- 0.1 microM, reversing the effect of the first phase. Due to energy coupling effects associated with these phases, the mean dissociation constant for binding of Ca2+ to the C-ter EF hand pair is increased approximately 3-fold, from 1.8 +/- 0.1 to 5.1 +/- 0.7 microM, and the mean dissociation constant for binding of Ca2+ to the N-ter EF hand pair is decreased by the same factor, from 11.2 +/- 1.0 to 3.5 +/- 0.6 microM. These characteristics produce a bell-shaped relationship between the apparent dissociation constant for the complex and the free Ca2+ concentration, with a distance of 5-6 microM between the midpoints of the rising and falling phases. Release of CaM from the neuromodulin IQ domain therefore appears to be promoted over a relatively narrow range of free Ca2+ concentrations. Our results demonstrate that CaM-IQ domain complexes can function as biphasic Ca2+ switches through opposing effects of Ca2+ bound sequentially to the two EF hand pairs in CaM.
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