ArticleWorld journal of gastroenterology2006
Identification of biomarkers of human pancreatic adenocarcinomas by expression profiling and validation with gene expression analysis in endoscopic ultrasound-guided fine needle aspiration samples.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- Multianalyte Serum Biomarker Panel for Early Detection of Pancreatic Adenocarcinoma.JCO clinical cancer informatics · 2023Article
- Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology.Frontiers in molecular biosciences · 2023Article
- Observational
- Review
- Essential thrombocythemia: a hemostatic view of thrombogenic risk factors and prognosis.Molecular biology reports · 2020Review
- Lipocalin-2 expression and function in pancreatic diseases.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] · 2020Review
- Implication and role of neutrophil gelatinase-associated lipocalin in cancer: lipocalin-2 as a potential novel emerging comprehensive therapeutic target for a variety of cancer types.Molecular biology reports · 2020Review
- New era for pancreatic endoscopic ultrasound: From imaging to molecular pathology of pancreatic cancer.World journal of gastrointestinal oncology · 2019Article
- On Predicting lung cancer subtypes using 'omic' data from tumor and tumor-adjacent histologically-normal tissue.BMC cancer · 2016Article
- Role of endoscopic ultrasound in the molecular diagnosis of pancreatic cancer.World journal of gastroenterology · 2014Review
- Targeting pancreatic cancer with magneto-fluorescent theranostic gold nanoshells.Nanomedicine (London, England) · 2014Article
- Tpl2 knockout keratinocytes have increased biomarkers for invasion and metastasis.Carcinogenesis · 2013Article
- Neutrophil gelatinase-associated lipocalin protein as a biomarker in the diagnosis of breast cancer: A meta-analysis.Biomedical reports · 2013Article
- Endoscopic ultrasound-guided fine needle aspiration: from the past to the future.Endoscopic ultrasound · 2013Review
- Extra- and intracellular imaging of human matrix metalloprotease 11 (hMMP-11) with a cell-penetrating FRET substrate.The Journal of biological chemistry · 2012Article
- The multifaceted roles of neutrophil gelatinase associated lipocalin (NGAL) in inflammation and cancer.Biochimica et biophysica acta · 2012Review
- Platelet-derived growth factor-C (PDGF-C) activation by serine proteases: implications for breast cancer progression.The Biochemical journal · 2012Article
- Advances in biomarker research for pancreatic cancer.Current pharmaceutical design · 2012Review
- Lipocalin2 promotes invasion, tumorigenicity and gemcitabine resistance in pancreatic ductal adenocarcinoma.PloS one · 2012Article
- Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo compare gene expression profiles of pancreatic adenocarcinoma tissue specimens, human pancreatic and colon adenocarcinoma and leukemia cell lines and normal pancreas samples in order to distinguish differentially expressed genes and to validate the differential expression of a subset of genes by quantitative real-time RT-PCR (RT-QPCR) in endoscopic ultrasound-guided fine needle aspiration (EUS-guided FNA) specimens.
methodsCommercially dedicated cancer cDNA macroarrays (Atlas Human Cancer 1.2) containing 1176 genes were used. Different statistical approaches (hierarchical clustering, principal component analysis (PCA) and SAM) were used to analyze the expression data. RT-QPCR and immunohistochemical studies were used for validation of results.
resultsRT-QPCR validated the increased expression of LCN2 (lipocalin 2) and for the first time PLAT (tissue-type plasminogen activator or tPA) in malignant pancreas as compared with normal pancreas. Immunohistochemical analysis confirmed the increased expression of LCN2 protein localized in epithelial cells of ducts invaded by carcinoma. The analysis of PLAT and LCN2 transcripts in 12 samples obtained through EUS-guided FNA from patients with pancreatic adenocarcinoma showed significantly increased expression levels in comparison with those found in normal tissues, indicating that a sufficient amount of high quality RNA can be obtained with this technique.
conclusionExpression profiling is a useful method to identify biomarkers and potential target genes. Molecular analysis of EUS-guided FNA samples in pancreatic cancer appears as a valuable strategy for the diagnosis of pancreatic adenocarcinomas.
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