ArticleCurrent microbiology2006
Gp63-like molecules in Phytomonas serpens: possible role in the insect interaction.
Article in Current microbiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Role of Virulence Factors of Trypanosomatids in the Insect Vector and Putative Genetic Events Involved in Surface Protein Diversity.Frontiers in cellular and infection microbiology · 2022Review
- Anti-Leishmania braziliensis activity of 1,10-phenanthroline-5,6-dione and its Cu(II) and Ag(I) complexes.Parasitology research · 2021Article
- The Enhanced Expression of Cruzipain-Like Molecules in the PhytoflagellateFrontiers in cellular and infection microbiology · 2021Article
- Natural infection by the protozoan Leptomonas wallacei impacts the morphology, physiology, reproduction, and lifespan of the insect Oncopeltus fasciatus.Scientific reports · 2019Article
- Participation of Trypanosoma cruzi gp63 molecules on the interaction with Rhodnius prolixus.Parasitology · 2019Article
- Dramatic changes in gene expression in different forms of Crithidia fasciculata reveal potential mechanisms for insect-specific adhesion in kinetoplastid parasites.PLoS neglected tropical diseases · 2019Article
- Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host.Memorias do Instituto Oswaldo Cruz · 2017Article
- Phytomonas: trypanosomatids adapted to plant environments.PLoS pathogens · 2015Review
- The streamlined genome of Phytomonas spp. relative to human pathogenic kinetoplastids reveals a parasite tailored for plants.PLoS genetics · 2014Article
- Transovum transmission of trypanosomatid cysts in the Milkweed bug, Oncopeltus fasciatus.PloS one · 2014Article
- Article
- Major surface protease of trypanosomatids: one size fits all?Infection and immunity · 2010Review
- Differential influence of gp63-like molecules in three distinct Leptomonas species on the adhesion to insect cells.Parasitology research · 2009Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we demonstrated that metallopeptidase inhibitors (EDTA, EGTA, and 1,10-phenanthroline) were able to arrest Phytomonas serpens growth in distinct patterns. This parasite released exclusively metallopeptidases to the extracellular environment, whereas in cellular extracts only cysteine peptidases were detected. In addition, an extracellular polypeptide of 60 kDa reacted in Western blotting probed with polyclonal antibody raised against gp63 of Leishmania amazonensis. In the cellular parasite extract, this antibody recognized bands migrating at 63 and 52 kDa, which partitioned on both aqueous and membrane-rich fractions. Flow cytometry and fluorescence microscopy analyses showed that the gp63-like molecules have a surface location. Moreover, phospholipase C (PLC)-treated parasites reduced the number of gp63-positive cells. The anti-cross-reacting determinant (CRD) and anti-gp63 antibodies recognized the 60-kDa band in the supernatant from PLC-treated cells, suggesting that this protein is glycosylphosphatidylinositol-anchored to the plasma membrane. This is the first report on the presence of gp63-like molecules in members of the Phytomonas genus. The pretreatment of the parasites with anti-gp63 antibody significantly diminished their adhesion index to explanted salivary glands of the phytophagous insect Oncopeltus fasciatus, suggesting a potential involvement of the gp63-like molecules in the adhesive process of this plant trypanosomatid.
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