ArticleBiophysical journal2006
Elastic response, buckling, and instability of microtubules under radial indentation.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Review
- Nano-Scale Video Imaging of Motility Machinery by High-Speed Atomic Force Microscopy.Biomolecules · 2025Review
- Mechanical fatigue in microtubules.Scientific reports · 2024Article
- Mechanical Properties of Viruses.Sub-cellular biochemistry · 2024Review
- The Influence of Nucleus Mechanics in Modelling Adhesion-independent Cell Migration in Structured and Confined Environments.Bulletin of mathematical biology · 2023Article
- Review
- The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin.Current biology : CB · 2022Article
- Feature Extraction of 3T3 Fibroblast Microtubule Based on Discrete Wavelet Transform and Lucy-Richardson Deconvolution Methods.Micromachines · 2022Article
- Dynamic Instability from Non-equilibrium Structural Transitions on the Energy Landscape of Microtubule.Cell systems · 2020Article
- Potent Small-Molecule Inhibitors Targeting Acetylated Microtubules as Anticancer Agents Against Triple-Negative Breast Cancer.Biomedicines · 2020Article
- Coarse-Grained Simulation of Mechanical Properties of Single Microtubules With Micrometer Length.Frontiers in molecular biosciences · 2020Article
- The AP2 adaptor enhances clathrin coat stiffness.The FEBS journal · 2019Article
- An estimate to the first approximation of microtubule rupture force.European biophysics journal : EBJ · 2019Article
- Article
- Direct visualization of single virus restoration after damage in real time.Journal of biological physics · 2018Article
- Article
- Computational modeling of single-cell mechanics and cytoskeletal mechanobiology.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- TensorCalculator: exploring the evolution of mechanical stress in the CCMV capsid.Journal of physics. Condensed matter : an Institute of Physics journal · 2018Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
- Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.Science (New York, N.Y.) · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We tested the mechanical properties of single microtubules by lateral indentation with the tip of an atomic force microscope. Indentations up to approximately 3.6 nm, i.e., 15% of the microtubule diameter, resulted in an approximately linear elastic response, and indentations were reversible without hysteresis. At an indentation force of around 0.3 nN we observed an instability corresponding to an approximately 1-nm indentation step in the taxol-stabilized microtubules, which could be due to partial or complete rupture of a relatively small number of lateral or axial tubulin-tubulin bonds. These indentations were reversible with hysteresis when the tip was retracted and no trace of damage was observed in subsequent high-resolution images. Higher forces caused substantial damage to the microtubules, which either led to depolymerization or, occasionally, to slowly reannealing holes in the microtubule wall. We modeled the experimental results using finite-element methods and find that the simple assumption of a homogeneous isotropic material, albeit structured with the characteristic protofilament corrugations, is sufficient to explain the linear elastic response of microtubules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.