ArticleThe Journal of cell biology1991
The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 85 citations in OpenAlex.
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- The flagellar length control system: exploring the physical biology of organelle size.Physical biology · 2023Review
- TK216 targets microtubules in Ewing sarcoma cells.Cell chemical biology · 2022Article
- Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations.PloS one · 2020Article
- The elusive actin cytoskeleton of a green alga expressing both conventional and divergent actins.Molecular biology of the cell · 2019Article
- Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium.Current biology : CB · 2019Article
- HeLife · 2017Article
- Flexural Rigidity and Shear Stiffness of Flagella Estimated from Induced Bends and Counterbends.Biophysical journal · 2016Article
- Quantitative analysis and modeling of katanin function in flagellar length control.Molecular biology of the cell · 2014Article
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- Mutations in alpha-tubulin confer dinitroaniline resistance at a cost to microtubule function.Molecular biology of the cell · 2007Article
- Molecular bases for sensitivity to tubulin-binding herbicides in green foxtail.Plant physiology · 2004Article
- A beta-tubulin mutation selectively uncouples nuclear division and cytokinesis in Tetrahymena thermophila.Eukaryotic cell · 2004Article
- Dinitroanilines bind alpha-tubulin to disrupt microtubules.Molecular biology of the cell · 2004Article
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- Plant tubulins: a melting pot for basic questions and promising applications.Transgenic research · 2000Review
- Molecular characterization of four beta-tubulin genes from dinitroaniline susceptible and resistant biotypes of Eleusine indica.Plant molecular biology · 1999Article
- Alpha-tubulin missense mutations correlate with antimicrotubule drug resistance in Eleusine indica.The Plant cell · 1998Article
- Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies.Genetics · 1995Article
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2 authors at 1 institution in 1 country.
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Abstract
The colR4 and colR15 beta 2-tubulin missense mutations for lysine-350 in Chlamydomonas reinhardtii (Lee and Huang, 1990) were originally isolated by selection for resistance to the growth inhibitory effects of colchicine. The colR4 and colR15 mutants have been found to be cross resistant to vinblastine and several classes of antimitotic herbicides, including the dinitroanilines (oryzalin, trifluralin, profluralin, and ethafluralin); the phosphoric amide amiprophos methyl; and the dimethyl propynl benzamide pronamide. Like colchicine and vinblastine, the antimitotic effects of these plant-specific herbicides have been associated with the depolymerization of microtubules. In contrast to their resistance to microtubule-depolymerizing drugs, the mutants have an increased sensitivity to taxol, a drug which enhances the polymerization and stability of microtubules. This pattern of altered sensitivity to different microtubule inhibitors was found to cosegregate and corevert with the beta-tubulin mutations providing the first genetic evidence that the in vivo herbicidal effects of the dinitroanilines, amiprophos methyl, and pronamide are related to microtubule function. Although wild-type like in their growth characteristics, the colR4 and colR15 mutants were found to have an altered pattern of microtubules containing acetylated alpha-tubulin, a posttranslational modification that has been associated with stable subsets of microtubules found in a variety of cells. Microtubules in the interphase cytoplasm and those of the intranuclear spindle of mitotic cells, which in wild-type Chlamydomonas cells do not contain acetylated alpha-tubulin, were found to be acetylated in the mutants. These data taken together suggest that the colR4 and colR15 missense mutations increase the stability of the microtubules into which the mutant beta-tubulins are incorporated and that the altered drug sensitivities of the mutants are a consequence of this enhanced microtubule stability.
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