ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2006
Detection of monkeypox virus with real-time PCR assays.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 236 papers, 2 of them syntheses that pooled it.
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Who cites it
236 citing papers in PubMed, 2 syntheses or guidelines pooled it, 476 citations in OpenAlex.
- Guidelines for the Laboratory Diagnosis of Monkeypox in Korea.Annals of laboratory medicine · 2023Guideline
- A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy.PLoS neglected tropical diseases · 2019Pooled it
- Comparative pathogenicity study of monkeypox virus Clade Ib and Clade IIb in a rabbit model.Virulence · 2026Article
- Development and clinical validation of a colorimetric isothermal assay for rapid detection of monkeypox virus.New microbes and new infections · 2026Article
- A comprehensive overview of monkeypox virus disease.Tropical medicine and health · 2026Review
- Community-based sentinel surveillance for Mpox-like disease among men who have sex with men and transgender individuals in India: a cross-sectional study.IJID regions · 2026Article
- Evaluation of a portable multiplex real-time pcr assay for the detection of monkeypox virus and non-variola orthopoxviruses.Virology journal · 2026Article
- A Global Health Alert on the Re-Emergence of Mpox a Viral Zoonotic Illness Its Prevention and Management: A Narrative Review.Health science reports · 2026Article
- Portable Detection of Monkeypox virus Clades using Bridging LAMP.Research square · 2026Article
- Orthopoxvirus Antibodies in Feral Mammals in Mpox Outbreak Areas, Nigeria, 2021-2022.Emerging infectious diseases · 2026Article
- Evaluation and Clinical Validation of Pan-Specific and Clade-Specific Diagnostic Real-Time PCR Assays for Monkeypox Virus.Journal of medical virology · 2026Article
- Monkeypox (Mpox), a Resurging Global Public Health Concern: An Updated Outlook Through 2025.Current issues in molecular biology · 2026Review
- Advanced strategies for the diagnosis, treatment, and vaccination of monkeypox.Journal of nanobiotechnology · 2026Review
- Development and evaluation of a two-tube multiplex real-time PCR assay for differential detection of Monkeypox virus clades Ia, Ib, IIa, and IIb.Frontiers in microbiology · 2026Article
- A retrospective comparative diagnostic evaluation of three commercial qPCR kits for the diagnosis of mpox virus on an enriched sample set.Frontiers in cellular and infection microbiology · 2026Article
- Unveiling Intra-Clonal Diversity of Monkeypox Virus from Brazil's First Outbreak Wave.Viruses · 2025Article
- Updated practice for detection of viral infections in breeding macaques.Zoological research · 2025Review
- Monkeypox Virus Partial-Genome Amplicon Sequencing for Improvement of Genomic Surveillance during Mpox Outbreaks.Emerging infectious diseases · 2025Article
- Patterns of genomic deletions in monkeypox virus during the 2022 outbreak in the United States.Nature communications · 2025Article
- When Acute Epstein-Barr Virus Mimics HIV: A Case of False-Positive p24 Antigen and Low-Level HIV Antibody Reactivity.Cureus · 2025Article
176 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman monkeypox, a zoonotic disease, was first reported outside of Africa during the 2003 US outbreak.
objectivesWe present two real-time PCR assays critical for laboratory diagnosis of monkeypox during the 2003 US outbreak. STUDY
designA TaqMan-based assay (E9L-NVAR) targets the orthopoxvirus DNA polymerase gene and detects Eurasian orthopoxviruses other than Variola. A hybridization assay, utilizing a MGB Eclipsetrade mark (Epoch Biosciences) probe, targets an envelope protein gene (B6R) and specifically detects monkeypox virus (MPXV). Assays were validated using coded orthopoxvirus DNA samples and used to evaluate lesion samples from five confirmed US monkeypox cases.
resultsE9L-NVAR did not detect variola (48 strains), North American orthopoxviruses (2), or DNA derived from non-poxviral rash illnesses. The assay reproducibly identified various concentrations of 13 Eurasian orthopoxvirus strains and was sensitive to 12.5 vaccinia genomes. The B6R assay recognized 15 different MPXV strains, while other orthopoxvirus (9) and bacteria (15) strains did not cross-react. Of the 13 human samples tested from confirmed cases, both assays identified 100% as containing MPXV DNA.
conclusionsE9L-NVAR and B6R assays demonstrate 100% specificity for non-variola Eurasian orthopoxvirus and MPXV, respectively. Using two discrete viral gene targets, these assays together provide a reliable and sensitive method for quickly confirming monkeypox infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.