ArticleJournal of molecular biology2006
Recognition and positioning of mRNA in the ribosome by tRNAs with expanded anticodons.
Article in Journal of molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Cellular Site-Specific Incorporation of Noncanonical Amino Acids in Synthetic Biology.Chemical reviews · 2024Review
- Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids.Chemical reviews · 2024Review
- Mechanisms and Delivery of tRNA Therapeutics.Chemical reviews · 2024Review
- Tuning tRNAs for improved translation.Frontiers in genetics · 2024Review
- Genetic Code Expansion Through Quadruplet Codon Decoding.Journal of molecular biology · 2022Review
- Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA.Nature communications · 2021Article
- Article
- Importance of a tRNA anticodon loop modification and a conserved, noncanonical anticodon stem pairing inThe Journal of biological chemistry · 2019Article
- Roles of specific aminoglycoside-ribosome interactions in the inhibition of translation.RNA (New York, N.Y.) · 2019Article
- Chimeric Translation for Mitochondrial Peptides: Regular and Expanded Codons.Computational and structural biotechnology journal · 2019Article
- Mechanism of tRNA-mediated +1 ribosomal frameshifting.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Bijective codon transformations show genetic code symmetries centered on cytosine's coding properties.Theory in biosciences = Theorie in den Biowissenschaften · 2018Article
- Structural insights into ribosome translocation.Wiley interdisciplinary reviews. RNA · 2016Review
- Epistasis analysis of 16S rRNA ram mutations helps define the conformational dynamics of the ribosome that influence decoding.RNA (New York, N.Y.) · 2016Article
- Systematic Evolution and Study of UAGN Decoding tRNAs in a Genomically Recoded Bacteria.Scientific reports · 2016Article
- Unbiased Mitoproteome Analyses Confirm Non-canonical RNA, Expanded Codon Translations.Computational and structural biotechnology journal · 2016Article
- Structural insights into translational recoding by frameshift suppressor tRNASufJ.RNA (New York, N.Y.) · 2014Article
- Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural determinants of an internal ribosome entry site that direct translational reading frame selection.Nucleic acids research · 2014Article
- A gripping tale of ribosomal frameshifting: extragenic suppressors of frameshift mutations spotlight P-site realignment.Microbiology and molecular biology reviews : MMBR · 2009Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mutant tRNAs containing an extra nucleotide in the anticodon loop are known to suppress +1 frameshift mutations, but in no case has the molecular mechanism been clarified. It has been proposed that the expanded anticodon pairs with a complementary mRNA sequence (the frameshift sequence) in the A site, and this quadruplet "codon-anticodon" helix is translocated to the P site to restore the correct reading frame. Here, we analyze the ability of tRNA analogs containing expanded anticodons to recognize and position mRNA in ribosomal complexes in vitro. In all cases tested, 8 nt anticodon loops position the 3' three-quarters of the frameshift sequence in the P site, indicating that the 5' bases of the expanded anticodon (nucleotides 33.5, 34, and 35) pair with mRNA in the P site. We also provide evidence that four base-pairs can form between the P-site tRNA and mRNA, and the fourth base-pair involves nucleotide 36 of the tRNA and lies toward (or in) the 30 S E site. In the A site, tRNA analogs with the expanded anticodon ACCG are able to recognize either CGG or GGU. These data imply a flexibility of the expanded anticodon in the A site. Recognition of the 5' three-quarters of the frameshift sequence in the A site and subsequent translocation of the expanded anticodon to the P site results in movement of mRNA by four nucleotides, explaining how these tRNAs can change the mRNA register in the ribosome to restore the correct reading frame.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.