ArticleBrain research2006
Upregulation of DMT1 expression in choroidal epithelia of the blood-CSF barrier following manganese exposure in vitro.
Article in Brain research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Cumulative and Interactive Effects of Heavy Metal Mixtures Across Neurodegenerative Diseases: A Comparative Review of Alzheimer, Parkinson, Amyotrophic Lateral Sclerosis and Multiple Sclerosis.Journal of xenobiotics · 2026Review
- Overlaps in mammalian iron and manganese homeostasis: recent advances.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Review
- Effects of Subchronic Manganese and Iron Exposure, Alone or in Combination, on Elemental Distribution in Rats.Biological trace element research · 2025Article
- Gut to brain: essential micronutrient and trace element manganese transport, function and toxicity.Frontiers in physiology · 2025Review
- Studies of Slc30a10 Deficiency in Mice Reveal That Intestinal Iron Transporters Dmt1 and Ferroportin Transport Manganese.Cellular and molecular gastroenterology and hepatology · 2025Article
- In Vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Applications in the Development and Research of (Neuro)Pharmaceuticals.Pharmaceutics · 2022Review
- Protective Effects of Sodium Para-aminosalicylic Acid on Manganese-Induced Damage in Rat Pancreas.Biological trace element research · 2021Article
- Manganese methionine hydroxy analog chelated affects growth performance, trace element deposition and expression of related transporters of broilers.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Article
- Effects of Vitamin C and Magnesium L-threonate Treatment on Learning and Memory in Lead-poisoned Mice.Journal of veterinary research · 2021Article
- Mechanisms of divalent metal toxicity in affective disorders.Toxicology · 2016Review
- Article
- Manganese Toxicity Upon Overexposure: a Decade in Review.Current environmental health reports · 2015Review
- Elevated adult neurogenesis in brain subventricular zone following in vivo manganese exposure: roles of copper and DMT1.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Manganese in human parenteral nutrition: considerations for toxicity and biomonitoring.Neurotoxicology · 2014Article
- Regulation of copper transport crossing brain barrier systems by Cu-ATPases: effect of manganese exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2014Article
- Involvement of CTR1 and ATP7A in lead (Pb)-induced copper (Cu) accumulation in choroidal epithelial cells.Toxicology letters · 2014Article
- Copper and copper proteins in Parkinson's disease.Oxidative medicine and cellular longevity · 2014Review
- Manganese homeostasis and transport.Metal ions in life sciences · 2013Article
- Protective effects of isothiocyanates on blood-CSF barrier disruption induced by oxidative stress.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Impact of manganese on and transfer across blood-brain and blood-cerebrospinal fluid barrier in vitro.The Journal of biological chemistry · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Divalent metal transporter 1 (DMT1), whose mRNA possesses a stem-loop structure in 3'-untranslated region, has been identified in most organs and responsible for transport of various divalent metal ions. Previous work from this laboratory has shown that manganese (Mn) exposure alters the function of iron regulatory protein (IRP) and increases iron (Fe) concentrations in the cerebrospinal fluid (CSF). This study was designed to test the hypothesis that Mn treatment, by acting on protein-mRNA binding between IRP and DMT1 mRNA, altered the expression of DMT1 in an immortalized choroidal epithelial Z310 cell line which was derived from rat choroid plexus epithelia, leading to a compartmental shift of Fe from the blood to the CSF. Immunocytochemistry confirmed the presence of DMT1 in Z310 cell. Following in vitro exposure to Mn at 100 microM for 24 and 48 h, the expression of DMT1 mRNA in Z310 cells was significantly increased by 45.4% (P < 0.05) and 78.1% (P < 0.01), respectively, as compared to controls. Accordingly, Western blot analysis revealed a significant increase of DMT1 protein concentrations at 48 h after Mn exposure (100 microM). Electrophoretic mobility shift assay (EMSA) showed that Mn exposure increased binding of IRP to DMT1 mRNA in cultured choroidal Z310 cells. Moreover, real-time RT-PCR revealed no changes in DMT1 heterogeneous nuclear RNA (hnRNA) levels following Mn exposure. These data suggest that Mn appears to stabilize the binding of IRP to DMT1 mRNA, thereby increasing the expression of DMT1. The facilitated transport of Fe by DMT1 at the blood-CSF barrier may partly contribute to Mn-induced neurodegenerative Parkinsonism.
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