ArticleBMC developmental biology2006
Positive autoregulation of the transcription factor Pax6 in response to increased levels of either of its major isoforms, Pax6 or Pax6(5a), in cultured cells.
Article in BMC developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Implementing a trilineage differentiation in the ReproTracker assay for improved teratogenicity assessment.Frontiers in toxicology · 2025Article
- Review
- Autoregulation of Pax6 in neuronal cells is mediated by Pax6(5a), Pax6(ΔPD), SPARC, and p53.Molecular biology reports · 2022Article
- Identification of the Wallenda JNKKK as an Alk suppressor reveals increased competitiveness of Alk-expressing cells.Scientific reports · 2020Article
- The Spectrum ofGenes · 2019Review
- Level of hydrogen peroxide affects expression and sub-cellular localization of Pax6.Molecular biology reports · 2018Article
- Pax6 is essential for the generation of late-born retinal neurons and for inhibition of photoreceptor-fate during late stages of retinogenesis.Developmental biology · 2017Article
- Regulation of cerebral cortical neurogenesis by the Pax6 transcription factor.Frontiers in cellular neuroscience · 2015Article
- SpliceNet: recovering splicing isoform-specific differential gene networks from RNA-Seq data of normal and diseased samples.Nucleic acids research · 2014Article
- Article
- 3T3 cell lines stably expressing Pax6 or Pax6(5a)--a new tool used for identification of common and isoform specific target genes.PloS one · 2012Article
- Novel lines of Pax6-/- embryonic stem cells exhibit reduced neurogenic capacity without loss of viability.BMC neuroscience · 2010Article
- The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.PLoS genetics · 2009Article
- Er81 is a downstream target of Pax6 in cortical progenitors.BMC developmental biology · 2008Article
- Controlled overexpression of Pax6 in vivo negatively autoregulates the Pax6 locus, causing cell-autonomous defects of late cortical progenitor proliferation with little effect on cortical arealization.Development (Cambridge, England) · 2007Article
- Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.Neural development · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPax6 is a transcription factor essential for normal development of the eyes and nervous system. It has two major isoforms, Pax6 and Pax6(5a), and the ratios between their expression levels vary within narrow limits. We tested the effects of overexpressing either one or other isoform on endogenous Pax6 expression levels in Neuro2A and NIH3T3 cells.
resultsWe found that both isoforms caused an up-regulation of endogenous Pax6 expression in cells with (Neuro2A) or without (NIH3T3) constitutive Pax6 expression. Western blots showed that cells stably transfected with constructs expressing either Pax6 or Pax6(5a) contained raised levels of both Pax6 and Pax6(5a). Quantitative RT-PCR confirmed an increase in levels of Pax6(5a) mRNA in cells containing Pax6-expressing constructs and an increase in levels of Pax6 mRNA in cells containing Pax6(5a)-expressing constructs. The fact that the introduction of constructs expressing only one isoform increased the cellular levels of not only that isoform but also the other indicates that activation of the endogenous Pax6 locus occurred. The ratio between the levels of the two isoforms was maintained close to physiological values. The overexpression of either isoform in neuroblastoma (Neuro2A) cell lines also promoted morphological change and an increase in beta-III-tubulin expression, indicating an increase in neurogenesis.
conclusionOur results demonstrate that Pax6 can up-regulate production of Pax6 protein from an entire intact endogenous Pax6 locus in its genomic environment. This adds to previous studies showing that Pax6 can up-regulate reporter expression driven by isolated Pax6 regulatory elements. Furthermore, our results suggest that an important function of positive feedback might be to stabilise the relative levels of Pax6 and Pax6(5a).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.