ArticleAntimicrobial agents and chemotherapy2006
DNA microarray for detection of macrolide resistance genes.
Article in Antimicrobial agents and chemotherapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 34 citations in OpenAlex.
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- Molecular Mechanisms of Drug Resistance inInternational journal of molecular sciences · 2022Review
- Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.Clinical microbiology reviews · 2016Review
- The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.Clinical microbiology reviews · 2015Review
- Molecular characterization of multidrug resistant hospital isolates using the antimicrobial resistance determinant microarray.PloS one · 2013Article
- Novel primer-probe sets for detection and identification of mycobacteria by PCR-microarray assay.Journal of clinical microbiology · 2012Article
- Persistent carriage and infection by multidrug-resistant Escherichia coli ST405 producing NDM-1 carbapenemase: report on the first Italian cases.Journal of clinical microbiology · 2011Article
- CEM-101 activity against Gram-positive organisms.Antimicrobial agents and chemotherapy · 2010Article
- Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.Microbial drug resistance (Larchmont, N.Y.) · 2010Article
- Integrated detection of extended-spectrum-beta-lactam resistance by DNA microarray-based genotyping of TEM, SHV, and CTX-M genes.Journal of clinical microbiology · 2010Article
- Development and application of the active surveillance of pathogens microarray to monitor bacterial gene flux.BMC microbiology · 2008Article
- Microbial Diagnostic Array Workstation (MDAW): a web server for diagnostic array data storage, sharing and analysis.Source code for biology and medicine · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A DNA microarray was developed to detect bacterial genes conferring resistance to macrolides and related antibiotics. A database containing 65 nonredundant genes selected from publicly available DNA sequences was constructed and used to design 100 oligonucleotide probes that could specifically detect and discriminate all 65 genes. Probes were spotted on a glass slide, and the array was reacted with DNA templates extracted from 20 reference strains of eight different bacterial species (Streptococcus pneumoniae, Streptococcus pyogenes, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Staphylococcus haemolyticus, Escherichia coli, and Bacteroides fragilis) known to harbor 29 different macrolide resistance genes. Hybridization results showed that probes reacted with, and only with, the expected DNA templates and allowed discovery of three unexpected genes, including msr(SA) in B. fragilis, an efflux gene that has not yet been described for gram-negative bacteria.
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