ArticleMolecular genetics and genomics : MGG2006
Genetic evidence for 18S rRNA binding and an Rps19p assembly function of yeast nucleolar protein Nep1p.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Specialized ribosomes: integrating new insights and current challenges.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Roles of ribosomal RNA in health and disease.Frontiers in RNA research · 2023Article
- Mutations in RPS19 may affect ribosome function and biogenesis in Diamond Blackfan anemia.FEBS open bio · 2022Article
- Chemical Modifications of Ribosomal RNA.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The ribosome epitranscriptome: inert-or a platform for functional plasticity?RNA (New York, N.Y.) · 2021Review
- Tuning the ribosome: The influence of rRNA modification on eukaryotic ribosome biogenesis and function.RNA biology · 2017Review
- Human diseases of the SSU processome.Biochimica et biophysica acta · 2014Review
- Mutation of EMG1 causing Bowen-Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay.BBA clinical · 2014Article
- Ribosome biogenesis in skeletal development and the pathogenesis of skeletal disorders.Biochimica et biophysica acta · 2014Review
- The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA.RNA (New York, N.Y.) · 2012Article
- Article
- Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis.Nucleic acids research · 2011Article
- The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA.Nucleic acids research · 2011Article
- EMG1 is essential for mouse pre-implantation embryo development.BMC developmental biology · 2010Article
- The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.Nucleic acids research · 2010Article
- Comprehensive molecular structure of the eukaryotic ribosome.Structure (London, England : 1993) · 2009Article
- Powering through ribosome assembly.RNA (New York, N.Y.) · 2009Review
- Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome.American journal of human genetics · 2009Article
- The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.Nucleic acids research · 2008Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleolar protein Nep1 and its human homologue were previously shown to be involved in the maturation of 18S rRNA and to interfere directly or indirectly with a methylation reaction. Here, we report that the loss-of-function mutation Deltasnr57 and multicopy expression of the ribosomal 40S subunit protein 19 (Rps19p) can partially suppress the Saccharomyces cerevisiae Deltanep1 growth defect. SnR57 mediates 2'-O-ribose-methylation of G(1570) in the 18S rRNA. By performing a three-hybrid screen, we isolated several short RNA sequences with strong binding affinity to Nep1p. All isolated RNAs shared a six-nucleotide consensus motif C/UUCAAC. Furthermore, one of the isolated RNAs exactly corresponded to nucleotides 1553-1577 of the 18S rRNA, which includes G(1570), the site of snR57-dependent 18S rRNA methylation. From protein-protein crosslink data and the cryo-EM map of the S. cerevisiae small ribosomal subunit, we suggest that Rps19p is localized in close vicinity to the Nep1p 18S rRNA binding site. Our results suggest that Nep1p binds adjacent to helix 47 of the 18S rRNA and possibly supports the association of Rps19p to pre-ribosomal particles.
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