ArticleMethods in molecular biology (Clifton, N.J.)2006
Laser scanning cytometry: principles and applications.
Article in Methods in molecular biology (Clifton, N.J.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Recent advances in tissue imaging for cancer research.F1000Research · 2019Review
- Review: imaging technologies for flow cytometry.Lab on a chip · 2016Review
- Review of methods to probe single cell metabolism and bioenergetics.Metabolic engineering · 2015Review
- Expression of the p12 subunit of human DNA polymerase δ (Pol δ), CDK inhibitor p21(WAF1), Cdt1, cyclin A, PCNA and Ki-67 in relation to DNA replication in individual cells.Cell cycle (Georgetown, Tex.) · 2014Article
- Laser scanning cytometry: principles and applications-an update.Methods in molecular biology (Clifton, N.J.) · 2013Review
- Imaging flow cytometry: coping with heterogeneity in biological systems.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
- Microtubule and cortical forces determine platelet size during vascular platelet production.Nature communications · 2012Article
- Genome protective effect of metformin as revealed by reduced level of constitutive DNA damage signaling.Aging · 2011Article
- Cell fixation in zinc salt solution is compatible with DNA damage response detection by phospho-specific antibodies.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Article
- Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on "click chemistry".Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Review
- Apoptosis and beyond: cytometry in studies of programmed cell death.Methods in cell biology · 2011Review
- Analysis of individual molecular events of DNA damage response by flow- and image-assisted cytometry.Methods in cell biology · 2011Review
- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Fluorochrome-labeled inhibitors of caspases: convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Laser scanning cytometry for automation of the micronucleus assay.Mutagenesis · 2011Review
- New biomarkers probing depth of cell senescence assessed by laser scanning cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Quantitative assessment of β-cell apoptosis and cell composition of isolated, undisrupted human islets by laser scanning cytometry.Transplantation · 2010Article
- Flow cytometry and laser scanning cytometry, a comparison of techniques.Journal of clinical monitoring and computing · 2010Review
- DNA damage response induced by exposure of human lung adenocarcinoma cells to smoke from tobacco- and nicotine-free cigarettes.Cell cycle (Georgetown, Tex.) · 2010Article
- Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The laser scanning cytometer (LSC) is the microscope-based cytofluorometer that offers a plethora of analytical capabilities. Multilaser-excited fluorescence emitted from individual cells is measured at several wavelength ranges, rapidly (up to 5000 cells/min), with high sensitivity and accuracy. The following applications of LSC are reviewed: (1) identification of cells that differ in degree of chromatin condensation (e.g., mitotic or apoptotic cells or lymphocytes vs granulocytes vs monocytes); (2) detection of translocation between cytoplasm vs nucleus or nucleoplasm vs nucleolus of regulatory molecules such as NF-kappaB, p53, or Bax; (3) semiautomatic scoring of micronuclei in mutagenicity assays; (4) analysis of fluorescence in situ hybridization; (5) enumeration and morphometry of nucleoli; (6) analysis of phenotype of progeny of individual cells in clonogenicity assay; (7) cell immunophenotyping; (8) visual examination, imaging, or sequential analysis of the cells measured earlier upon their relocation, using different probes; (9) in situ enzyme kinetics and other time-resolved processes; (10) analysis of tissue section architecture; (11) application for hypocellular samples (needle aspirate, spinal fluid, etc.); (12) other clinical applications. Advantages and limitations of LSC are discussed and compared with flow cytometry.
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