ArticleWorld journal of gastroenterology2006
Production of reactive oxygen species and expression of inducible nitric oxide synthase in rat isolated Kupffer cells stimulated by Leptospira interrogans and Borrelia burgdorferi.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- A historical perspective of Kupffer cells in the context of infection.Cell and tissue research · 2025Review
- Advances in Understanding Lipopolysaccharide-Mediated Hepatitis: Mechanisms and Pathological Features.Current issues in molecular biology · 2025Review
- The oxidative stress response of pathogenic Leptospira is controlled by two peroxide stress regulators which putatively cooperate in controlling virulence.PLoS pathogens · 2021Article
- The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress.PLoS pathogens · 2020Article
- Structure and function of theThe Journal of biological chemistry · 2018Article
- New progress in roles of nitric oxide during hepatic ischemia reperfusion injury.World journal of gastroenterology · 2017Review
- The role of reactive oxygen intermediates in the intracellular fate of Leptospira interrogans in the macrophages of different hosts.PloS one · 2017Article
- Infection of human monocytes by Chlamydia pneumoniae and Chlamydia trachomatis: an in vitro comparative study.BMC research notes · 2014Article
- Article
- Leptospira interrogans catalase is required for resistance to H2O2 and for virulence.Infection and immunity · 2012Article
- Production of reactive oxygen (H2O2) and nitrogen (NO) intermediates and tnf-α in mice genetically selected for high (H) and low (L) antibody response and experimentally infected with Leptospira serovar pomona.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2011Article
- CD14 signaling restrains chronic inflammation through induction of p38-MAPK/SOCS-dependent tolerance.PLoS pathogens · 2009Article
- Chlamydial and periodontal pathogens induce hepatic inflammation and fatty acid imbalance in apolipoprotein E-deficient mice.Infection and immunity · 2009Article
- Serum sialic acid and oxidative stress parameters changes in cattle with leptospirosis.Veterinary research communications · 2008Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the production of reactive oxygen species (ROS) and the expression of inducible nitric oxide synthase (iNOS) in rat isolated Kupffer cells (KCs) stimulated by Leptospira interrogans and Borrelia burgdorferi.
methodsRat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Purified Kupffer cells were tested in vitro with alive L. interogans and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies.
resultsB. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection.
conclusionBoth genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.
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