ArticlePlanta2006
EST generation and analyses towards identifying female gametophyte-specific genes in Zea mays L.
Article in Planta, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 83 citations in OpenAlex.
- Embryo Rescue in Plant Breeding.Plants (Basel, Switzerland) · 2023Review
- Isolation of gametes and zygotes from Setaria viridis.Journal of plant research · 2022Article
- Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes.Bio-protocol · 2020Article
- Overlapping Patterns of Gene Expression Between Gametophyte and Sporophyte Phases in the FernFrontiers in plant science · 2018Article
- Development of gene expression system in egg cells and zygotes isolated from rice and maize.Plant direct · 2017Article
- SUPPRESSOR OF FRIGIDA (SUF4) Supports Gamete Fusion via Regulating Arabidopsis EC1 Gene Expression.Plant physiology · 2017Article
- Patterning the Female Gametophyte of Flowering Plants.Plant physiology · 2017Review
- Bioinformatics resources for pollen.Plant reproduction · 2016Review
- Versatility of germin-like proteins in their sequences, expressions, and functions.Functional & integrative genomics · 2015Article
- The male germline of angiosperms: repertoire of an inconspicuous but important cell lineage.Frontiers in plant science · 2015Review
- Discovery of novel transcripts and gametophytic functions via RNA-seq analysis of maize gametophytic transcriptomes.Genome biology · 2014Article
- Transcript profiles of maize embryo sacs and preliminary identification of genes involved in the embryo sac-pollen tube interaction.Frontiers in plant science · 2014Article
- Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.).BMC genomics · 2013Article
- Gene expression profiles in rice gametes and zygotes: identification of gamete-enriched genes and up- or down-regulated genes in zygotes after fertilization.Journal of experimental botany · 2013Article
- Members of the germin-like protein family in Brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of Sclerotinia sclerotiorum.Journal of experimental botany · 2012Article
- Transcriptional activity of Hyacinthus orientalis L. female gametophyte cells before and after fertilization.Planta · 2012Article
- Nuclear behavior, cell polarity, and cell specification in the female gametophyte.Sexual plant reproduction · 2011Review
- Analysis of stunter1, a maize mutant with reduced gametophyte size and maternal effects on seed development.Genetics · 2011Article
- Distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays.Plant physiology · 2011Article
- The female gametophyte.The arabidopsis book · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The embryo sac (female gametophyte) plays an important role in double fertilization. The female gametophyte is composed of four specific cell types: the synergids that attract pollen tubes, the egg cell and central cell which are fusion partners for the two sperm cells, and the antipodal cells whose function is unknown. As a resource for gene discovery and to help identify genes exhibiting cell-specific expression patterns, we constructed cDNA libraries from female gametophytes and from egg cells of maize and sequenced more than 8,500 ESTs. These libraries represent diverse transcripts, potentially corresponding to 3,850 genes (contigs and singletons) from the female gametophyte and 963 genes (contigs and singletons) from the egg cell. In each collection, 16% of the contigs/singletons have no matches in databases and 3-5% encode hypothetical proteins; novel hypothetical proteins (not found within the female gametophyte contigs) were identified among the egg cell contigs. We examined 65 contigs by RT-PCR and 19 genes that were potentially female gametophyte-specific were identified. We used in situ hybridization to determine expression specificity for seven genes: one transcript was expressed both in the egg cell and in the central cell, one was expressed in the egg cell and synergids, two were expressed in the central cell, two were expressed in the synergids, and one was expressed in the central cell and the synergids. Four of these encode small, potentially secreted peptides that are dissimilar except for a conserved triple cysteine motif near their C-terminus. These EST resources should prove useful for identifying female gametophyte or cell-specific genes.
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