ArticleMolecular and cellular biochemistry2006
Pluripotency potential of human adipose-derived stem cells marked with exogenous green fluorescent protein.
Article in Molecular and cellular biochemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Exosomes from human induced pluripotent stem cells derived mesenchymal stem cells improved myocardial injury caused by severe acute pancreatitis through activating Akt/Nrf2/HO-1 axis.Cell cycle (Georgetown, Tex.) · 2022Article
- MiR-26a regulated adipogenic differentiation of ADSCs induced by insulin through CDK5/FOXC2 pathway.Molecular and cellular biochemistry · 2021Article
- [Chondrocyte cocultures with stromal vascular fraction of adipose tissue promote cartilage regeneration in vivo].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2020Article
- Adipose-derived Stem/Stromal Cells on Electrospun Fibrin Microfiber Bundles Enable Moderate Muscle Reconstruction in a Volumetric Muscle Loss Model.Cell transplantation · 2018Article
- MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling.Scientific reports · 2014Article
- Therapeutic potential of human adipose-derived stem cells (ADSCs) from cancer patients: a pilot study.PloS one · 2014Article
- Uniaxial cyclic strain of human adipose-derived mesenchymal stem cells and C2C12 myoblasts in coculture.Journal of tissue engineering · 2014Article
- Effects of bone morphogenetic protein-4 (BMP-4) on adipocyte differentiation from mouse adipose-derived stem cells.Cell proliferation · 2013Article
- Secreted factors from adipose tissue increase adipogenic differentiation of mesenchymal stem cells.Cell proliferation · 2012Article
- Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.Veterinary research communications · 2011Article
- Adipose derived stem cells: efficiency, toxicity, stability of BrdU labeling and effects on self-renewal and adipose differentiation.Molecular and cellular biochemistry · 2011Article
- In vitro osteogenic differentiation of adipose stem cells after lentiviral transduction with green fluorescent protein.The Journal of craniofacial surgery · 2009Article
- Adult stromal cells derived from human adipose tissue provoke pancreatic cancer cell death both in vitro and in vivo.PloS one · 2009Article
- Identification of osteo-adipo progenitor cells in fat tissue.Cell proliferation · 2008Article
- Bone regeneration by BMP-2 enhanced adipose stem cells loading on alginate gel.Histochemistry and cell biology · 2008Article
- Expression of Pcp4 gene during osteogenic differentiation of bone marrow mesenchymal stem cells in vitro.Molecular and cellular biochemistry · 2008Article
- Recent progress on tissue-resident adult stem cell biology and their therapeutic implications.Stem cell reviews · 2008Review
- Regulation of skeletal muscle differentiation in fibroblasts by exogenous MyoD gene in vitro and in vivo.Molecular and cellular biochemistry · 2007Article
- Osteogenic differentiation of adipose derived stem cells promoted by overexpression of osterix.Molecular and cellular biochemistry · 2007Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Musculoskeletal tissues regeneration requires rapid expansion of seeding cells both in vitro and in vivo while maintaining their multilineage differentiation ability. Human adipose-derived stem cells (ASCs) are considered to contain multipotent mesenchymal stem cells. Monolayer cultures of human ASCs were isolated from human lipoaspirates and passaged 3 times and then infected with replication-incompetent adenoviral vectors carrying green fluorescent protein (Ad/GFP) genes. Then, Ad/GFP infected human ASCs were transferred to osteogenic, chondrogenic, adipogenic, and myogenic medium. The morphological characterization of induced cells was observed using phase-contrast microscopy and fluorescence microscopy. The expression of marker proteins or genes was measured by immunocytochemical and RT-PCR analysis. Osteopontin (OPN), and osteocalcin (OCN) were positive in osteogenic lineages, aggrecan and SOX9 were positive in chondrogenic ones, peroxisome proliferator-activated receptor (PPAR-gamma2) and lipoprotein lipase (LPL) were positive in adipogenic ones, and myogenin and myod1 was positive in myogenic ones. At the same time, the results of fluorescence microscopic imaging proved that the high level of GFP expression during ASCs differentiation maintained stable nearly 2 months. So the exogenous GFP and multilineage potential of human ASCs had no severe influences on each other. Since the human ASCs can be easily obtained and abundant, it is proposed that they may be promising candidate cells for further studies on tissue engineering. Imaging with expression of GFP facilitates the research on ASCs physiological behavior and application in tissue engineering during differentiation both in vitro and in vivo.
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