ArticleThe Plant cell2006
Targeting of the plant vacuolar sorting receptor BP80 is dependent on multiple sorting signals in the cytosolic tail.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 96 citations in OpenAlex.
- A Sugarcane Smut Fungus Effector Hijacks Plant Vacuolar Sorting Receptor-Mediated Trafficking to Evade Host Immune Detection.Plant, cell & environment · 2025Article
- Studying Secretory Protein Synthesis in Nicotiana benthamiana Protoplasts.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The K/HDEL receptor does not recycle but instead acts as a Golgi-gatekeeper.Nature communications · 2023Article
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- Structural insights into how vacuolar sorting receptors recognize the sorting determinants of seed storage proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Motif-based endomembrane trafficking.Plant physiology · 2021Article
- Article
- An Arabidopsis protoplast isolation method reduces cytosolic acidification and activation of the chloroplast stress sensor SENSITIVE TO FREEZING 2.Plant signaling & behavior · 2019Article
- Predominant Golgi Residency of the Plant K/HDEL Receptor Is Essential for Its Function in Mediating ER Retention.The Plant cell · 2018Article
- Nanobody-triggered lockdown of VSRs reveals ligand reloading in the Golgi.Nature communications · 2018Article
- The Multivesicular Body and Autophagosome Pathways in Plants.Frontiers in plant science · 2018Article
- pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in Arabidopsis.The Plant cell · 2015Article
- Endosomal Interactions during Root Hair Growth.Frontiers in plant science · 2015Article
- Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor.The Journal of biological chemistry · 2014Article
- Article
- Vacuolar Sorting Receptor-Mediated Trafficking of Soluble Vacuolar Proteins in Plant Cells.Plants (Basel, Switzerland) · 2014Review
- The Arabidopsis Endosomal Sorting Complex Required for Transport III Regulates Internal Vesicle Formation of the Prevacuolar Compartment and Is Required for Plant Development.Plant physiology · 2014Article
- Activation of the Rab7 GTPase by the MON1-CCZ1 Complex Is Essential for PVC-to-Vacuole Trafficking and Plant Growth in Arabidopsis.The Plant cell · 2014Article
- Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco.The Plant cell · 2014Article
- Receptor-mediated transport of vacuolar proteins: a critical analysis and a new model.Protoplasma · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Although signals for vacuolar sorting of soluble proteins are well described, we have yet to learn how the plant vacuolar sorting receptor BP80 reaches its correct destination and recycles. To shed light on receptor targeting, we used an in vivo competition assay in which a truncated receptor (green fluorescent protein-BP80) specifically competes with sorting machinery and causes hypersecretion of BP80-ligands from tobacco (Nicotiana tabacum) leaf protoplasts. We show that both the transmembrane domain and the cytosolic tail of BP80 contain information necessary for efficient progress to the prevacuolar compartment (PVC). Furthermore, the tail must be exposed on the correct membrane surface to compete with sorting machinery. Mutational analysis of conserved residues revealed that multiple sequence motifs are necessary for competition, one of which is a typical Tyr-based motif (YXXPhi). Substitution of Tyr-612 for Ala causes partial retention in the Golgi apparatus, mistargeting to the plasma membrane (PM), and slower progress to the PVC. A role in Golgi-to-PVC transport was confirmed by generating the corresponding mutation on full-length BP80. The mutant receptor was partially mistargeted to the PM and induced the secretion of a coexpressed BP80-ligand. Further mutants indicate that the cytosolic tail is likely to contain other information besides the YXXPhi motif, possibly for endoplasmic reticulum export, endocytosis from the PM, and PVC-to-Golgi recycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.