ArticleRNA (New York, N.Y.)2006
Yeast transcripts cleaved by an internal ribozyme provide new insight into the role of the cap and poly(A) tail in translation and mRNA decay.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 95 citations in OpenAlex.
- The function of mRNA quality control in aging and age-related diseases.The Journal of biological chemistry · 2026Review
- Rational development of FMN-based orthogonal riboswitch that functions in response to specific non-cognate ligand.Nature communications · 2025Article
- Ending a bad start: Triggers and mechanisms of co-translational protein degradation.Frontiers in molecular biosciences · 2022Review
- A cell-based ribozyme reporter system employing a chromosomally-integrated 5' exonuclease gene.BMC molecular and cell biology · 2021Article
- Coupling of translation quality control and mRNA targeting to stress granules.The Journal of cell biology · 2020Article
- Detection and Degradation of Stalled Nascent Chains via Ribosome-Associated Quality Control.Annual review of biochemistry · 2020Review
- Optimization of 5' Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.Molecular therapy. Methods & clinical development · 2020Article
- Quality controls induced by aberrant translation.Nucleic acids research · 2020Review
- No-Go Decay mRNA cleavage in the ribosome exit tunnel produces 5'-OH ends phosphorylated by Trl1.Nature communications · 2020Article
- A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression inFrontiers in cellular and infection microbiology · 2020Article
- Massively parallel RNA device engineering in mammalian cells with RNA-Seq.Nature communications · 2019Article
- PolyA tracks, polybasic peptides, poly-translational hurdles.Wiley interdisciplinary reviews. RNA · 2018Review
- Vms1p is a release factor for the ribosome-associated quality control complex.Nature communications · 2018Article
- A Riboswitch-based Inducible Gene Expression System for Trypanosoma brucei.The Journal of eukaryotic microbiology · 2018Article
- Highly motif- and organism-dependent effects of naturally occurring hammerhead ribozyme sequences on gene expression.RNA biology · 2018Article
- Lariat capping as a tool to manipulate the 5' end of individual yeast mRNA species in vivo.RNA (New York, N.Y.) · 2017Article
- Asc1, Hel2, and Slh1 couple translation arrest to nascent chain degradation.RNA (New York, N.Y.) · 2017Article
- Translation of poly(A) tails leads to precise mRNA cleavage.RNA (New York, N.Y.) · 2017Article
- Ribosome-based quality control of mRNA and nascent peptides.Wiley interdisciplinary reviews. RNA · 2017Review
- The organization and regulation of mRNA-protein complexes.Wiley interdisciplinary reviews. RNA · 2017Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
It has been proposed that the 7-methylguanosine cap and poly(A) tail of mRNAs have important functions in translation and transcript stability. To directly test these roles of the cap and poly(A) tail, we have constructed plasmids with a ribozyme within the coding region or 3' UTR of reporter genes. We show that the unadenylated 5' cleavage product is translated and is rapidly degraded by the cytoplasmic exosome. This exosome-mediated decay is independent of the nonstop mRNA decay pathway, and, thus, reveals an additional substrate for exosome-mediated decay that may have physiological equivalents. The rapid decay of this transcript in the cytoplasm indicates that this unadenylated cleavage product is rapidly exported from the nucleus. We also show that this cleavage product is not subject to rapid decapping; thus, the lack of a poly(A) tail does not always trigger rapid decapping of the transcript. We show that the 3' cleavage product is rapidly degraded by Xrn1p in the cytoplasm. We cannot detect any protein from this 3' cleavage product, which supports previous data concluding that the 5' cap is required for translation. The reporter genes we have utilized in these studies should be generally useful tools in studying the importance of the poly(A) tail and 5' cap of a transcript for export, translation, mRNA decay, and other aspects of mRNA metabolism in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.