ArticleThe EMBO journal2006
Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA.
Article in The EMBO journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 101 citations in OpenAlex.
- G-quadruplex structures as regulators of cellular processes and drivers of genome instability in cancer.Critical reviews in biochemistry and molecular biology · 2026Review
- The Roles of Topoisomerases in Transcriptional Regulation.International journal of molecular sciences · 2026Review
- Mitotic genome folding.The Journal of cell biology · 2025Review
- Investigation of in-vivo and in-silico toxicity induced by environmental drug contamination in a non-target organism.Scientific reports · 2025Article
- Electrophoretic Analysis of DNA Supercoiling Activities.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Enzymatic Processing of DNA-Protein Crosslinks.Genes · 2024Review
- Chromatinization modulates topoisomerase II processivity.Nature communications · 2023Article
- Chromatinization Modulates Topoisomerase II Processivity.bioRxiv : the preprint server for biology · 2023Article
- TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cells.STAR protocols · 2022Article
- In vitro radiosensitization of breast cancer with hypoxia-activated prodrugs.Journal of cellular and molecular medicine · 2022Article
- CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae.FEMS yeast research · 2022Article
- Human topoisomerases and their roles in genome stability and organization.Nature reviews. Molecular cell biology · 2022Review
- The regulation of DNA supercoiling across evolution.Protein science : a publication of the Protein Society · 2021Review
- Topoisomerase IIβ targets DNA crossovers formed between distant homologous sites to induce chromatin opening.Scientific reports · 2020Article
- Chromatin Architectural Factors as Safeguards against Excessive Supercoiling during DNA Replication.International journal of molecular sciences · 2020Review
- Nanomotion detection based on atomic force microscopy cantilevers.Cell surface (Amsterdam, Netherlands) · 2019Review
- Article
- Transcriptional supercoiling boosts topoisomerase II-mediated knotting of intracellular DNA.Nucleic acids research · 2019Article
- Transfer-matrix calculations of the effects of tension and torque constraints on DNA-protein interactions.Nucleic acids research · 2018Article
- The Roles of DNA Topoisomerase IIβ in Transcription.International journal of molecular sciences · 2018Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic topoisomerases I and II efficiently remove helical tension in naked DNA molecules. However, this activity has not been examined in nucleosomal DNA, their natural substrate. Here, we obtained yeast minichromosomes holding DNA under (+) helical tension, and incubated them with topoisomerases. We show that DNA supercoiling density can rise above +0.04 without displacement of the histones and that the typical nucleosome topology is restored upon DNA relaxation. However, in contrast to what is observed in naked DNA, topoisomerase II relaxes nucleosomal DNA much faster than topoisomerase I. The same effect occurs in cell extracts containing physiological dosages of topoisomeraseI and II. Apparently, the DNA strand-rotation mechanism of topoisomerase I does not efficiently relax chromatin, which imposes barriers for DNA twist diffusion. Conversely, the DNA cross-inversion mechanism of topoisomerase II is facilitated in chromatin, which favor the juxtaposition of DNA segments. We conclude that topoisomerase II is the main modulator of DNA topology in chromatin fibers. The nonessential topoisomerase I then assists DNA relaxation where chromatin structure impairs DNA juxtaposition but allows twist diffusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.