ArticleGene therapy2006
Inhibition of human immunodeficiency virus type 1 by RNA interference using long-hairpin RNA.
Article in Gene therapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 67 citations in OpenAlex.
- Block-And-Lock: New Horizons for a Cure for HIV-1.Viruses · 2020Review
- Bone Marrow Gene Therapy for HIV/AIDS.Viruses · 2015Review
- Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100.BMC biotechnology · 2012Article
- Selection of RNAi-based inhibitors for anti-HIV gene therapy.World journal of virology · 2012Review
- A direct comparison of strategies for combinatorial RNA interference.BMC molecular biology · 2010Article
- Novel RNA-based strategies for therapeutic gene silencing.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Review
- Combinatorial RNAi against HIV-1 using extended short hairpin RNAs.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Trafficking through the Rev/RRE pathway is essential for efficient inhibition of human immunodeficiency virus type 1 by an antisense RNA derived from the envelope gene.Journal of virology · 2009Article
- Nucleic acids-based therapeutics in the battle against pathogenic viruses.Handbook of experimental pharmacology · 2009Review
- Progress in the therapeutic applications of siRNAs against HIV-1.Methods in molecular biology (Clifton, N.J.) · 2009Review
- A role for the Dicer helicase domain in the processing of thermodynamically unstable hairpin RNAs.Nucleic acids research · 2008Article
- Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistron.Nucleic acids research · 2008Article
- Increased virus replication in mammalian cells by blocking intracellular innate defense responses.Gene therapy · 2008Article
- Lentiviral vector design for multiple shRNA expression and durable HIV-1 inhibition.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- RNA interference against viruses: strike and counterstrike.Nature biotechnology · 2007Review
- Endoribonuclease-prepared short interfering RNAs induce effective and specific inhibition of human immunodeficiency virus type 1 replication.Journal of virology · 2007Article
- Progress and prospects: RNA-based therapies for treatment of HIV infection.Gene therapy · 2007Review
- Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites.Retrovirology · 2007Article
- Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome.Retrovirology · 2007Article
- Design of extended short hairpin RNAs for HIV-1 inhibition.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibition of virus replication by means of RNA interference has been reported for several important human pathogens, including human immunodeficiency virus type 1 (HIV-1). RNA interference against these pathogens has been accomplished by introduction of virus-specific synthetic small interfering RNAs (siRNAs) or DNA constructs encoding short-hairpin RNAs (shRNAs). Their use as therapeutic antiviral against HIV-1 is limited, because of the emergence of viral escape mutants. In order to solve this durability problem, we tested DNA constructs encoding virus-specific long-hairpin RNAs (lhRNAs) for their ability to inhibit HIV-1 production. Expression of lhRNAs in mammalian cells may result in the synthesis of many siRNAs targeting different viral sequences, thus providing more potent inhibition and reducing the chance of viral escape. The lhRNA constructs were compared with in vitro diced double-stranded RNA and a DNA construct encoding an effective nef-specific shRNA for their ability to inhibit HIV-1 production in cells. Our results show that DNA constructs encoding virus-specific lhRNAs are capable of inhibiting HIV-1 production in a sequence-specific manner, without inducing the class I interferon genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.