ArticleThe Journal of cell biology1991
Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 120 citations in OpenAlex.
- Celebrating the creative scientific life of Ken Jacobson.Biophysical journal · 2023Article
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS.Journal of physics D: Applied physics · 2017Review
- Single cell optical imaging and spectroscopy.Chemical reviews · 2013Review
- Fluorescence techniques to study lipid dynamics.Cold Spring Harbor perspectives in biology · 2011Review
- Effects of macromolecular crowding on intracellular diffusion from a single particle perspective.Biophysical reviews · 2010Article
- Both MHC class II and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking.Biophysical journal · 2008Article
- Cellular binding, motion, and internalization of synthetic gene delivery polymers.Biochimica et biophysica acta · 2007Article
- Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking.Biophysical journal · 2003Article
- Rearrangement of integrins in avidity regulation by leukocytes.Immunologic research · 2002Review
- A new dimension in retrograde flow: centripetal movement of engulfed particles.Biophysical journal · 2001Article
- Vesicle trafficking and cell surface membrane patchiness.Biophysical journal · 2001Article
- Activation-enhanced alpha(IIb)beta(3)-integrin-cytoskeleton interactions outside of focal contacts require the alpha-subunit.Molecular biology of the cell · 2001Article
- Calcium influx alters actin bundle dynamics and retrograde flow in Helisoma growth cones.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Weak dependence of mobility of membrane protein aggregates on aggregate size supports a viscous model of retardation of diffusion.Biophysical journal · 1999Article
- Cytoplasmic regulation of the movement of E-cadherin on the free cell surface as studied by optical tweezers and single particle tracking: corralling and tethering by the membrane skeleton.The Journal of cell biology · 1998Article
- Structural mosaicism on the submicron scale in the plasma membrane.Biophysical journal · 1998Article
- The silver anniversary of gold: 25 years of the colloidal gold marker system for immunocytochemistry and histochemistry.Histochemistry and cell biology · 1996Review
- Constrained diffusion or immobile fraction on cell surfaces: a new interpretation.Biophysical journal · 1996Article
- Adhesion-activating phorbol ester increases the mobility of leukocyte integrin LFA-1 in cultured lymphocytes.The Journal of clinical investigation · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recently introduced extension of video-enhanced light microscopy, called Nanovid microscopy, documents the dynamic reorganization of individual cell surface components on living cells. 40-microns colloidal gold probes coupled to different types of poly-L-lysine label negative cell surface components of PTK2 cells. Evidence is provided that they bind to negative sialic acid residues of glycoproteins, probably through nonspecific electrostatic interactions. The gold probes, coupled to short poly-L-lysine molecules (4 kD) displayed Brownian motion, with a diffusion coefficient in the range 0.1-0.2 micron2/s. A diffusion coefficient in the 0.1 micron2/s range was also observed with 40-nm gold probes coupled to an antibody against the lipid-linked Thy-1 antigen on 3T3 fibroblasts. Diffusion of these probes is largely confined to apparent microdomains of 1-2 microns in size. On the other hand, the gold probes, coupled to long poly-L-lysine molecules (240 kD) molecules and bound to the leading lamella, were driven rearward, toward the boundary between lamelloplasm and perinuclear cytoplasm at a velocity of 0.5-1 micron/min by a directed ATP-dependent mechanism. This uniform motion was inhibited by cytochalasin, suggesting actin microfilament involvement. A similar behavior on MO cells was observed when the antibody-labeled gold served as a marker for the PGP-1 (GP-80) antigen. These results show that Nanovid microscopy, offering the possibility to observe the motion of individual specific cell surface components, provides a new and powerful tool to study the dynamic reorganization of the cell membrane during locomotion and in other biological contexts as well.
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