ArticleThe Journal of cell biology1991
Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 111 citations in OpenAlex.
- Keeping a lid on nodal: transcriptional and translational repression of nodal signalling.Open biology · 2016Review
- PrimPol bypasses UV photoproducts during eukaryotic chromosomal DNA replication.Molecular cell · 2013Article
- CDC-48/p97 coordinates CDT-1 degradation with GINS chromatin dissociation to ensure faithful DNA replication.Molecular cell · 2011Article
- Cross-species hybridizations on a multi-species cDNA microarray to identify evolutionarily conserved genes expressed in oocytes.BMC genomics · 2006Article
- CAR-1 and trailer hitch: driving mRNP granule function at the ER?The Journal of cell biology · 2006Review
- Article
- Large-scale quantitative analysis of sources of variation in the actin polymerization-based movement of Listeria monocytogenes.Biophysical journal · 2005Article
- The major mRNA-associated protein YB-1 is a potent 5' cap-dependent mRNA stabilizer.The EMBO journal · 2001Article
- The RNA binding protein YB-1 binds A/C-rich exon enhancers and stimulates splicing of the CD44 alternative exon v4.The EMBO journal · 2001Article
- RNA-dependent cytoplasmic anchoring of a transcription factor subunit during Xenopus development.The EMBO journal · 2000Article
- Chicken Y-box proteins chk-YB-1b and chk-YB-2 repress translation by sequence-specific interaction with single-stranded RNA.The Biochemical journal · 2000Article
- Dual roles of p82, the clam CPEB homolog, in cytoplasmic polyadenylation and translational masking.RNA (New York, N.Y.) · 1999Article
- Overexpression in COS cells of p50, the major core protein associated with mRNA, results in translation inhibition.Nucleic acids research · 1997Article
- The RNA-binding protein, TB-RBP, is the mouse homologue of translin, a recombination protein associated with chromosomal translocations.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Characterization of a beta-actin mRNA zipcode-binding protein.Molecular and cellular biology · 1997Article
- Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes.Nucleic acids research · 1997Article
- Translation of a testis-specific Cu/Zn superoxide dismutase (SOD-1) mRNA is regulated by a 65-kilodalton protein which binds to its 5' untranslated region.Molecular and cellular biology · 1996Article
- Review
- Overexpression of poly(A) binding protein prevents maturation-specific deadenylation and translational inactivation in Xenopus oocytes.The EMBO journal · 1996Article
- Binding of Y-box proteins to RNA: involvement of different protein domains.Nucleic acids research · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To gain insight into the mechanisms involved in the formation of maternally stored mRNPs during Xenopus laevis development, we searched for soluble cytoplasmic proteins of the oocyte that are able to selectively bind mRNAs, using as substrate radiolabeled mRNA. In vitro mRNP assembly in solution was followed by UV-cross-linking and RNase digestion, resulting in covalent tagging of polypeptides by nucleotide transfer. Five polypeptides of approximately 54, 56 60, 70, and 100 kD (p54, p56, p60, p70, and p100) have been found to selectively bind mRNA and assemble into mRNPs. These polypeptides, which correspond to previously described native mRNP components, occur in three different particle classes of approximately 4.5S, approximately 6S, and approximately 15S, as also determined by their reactions with antibodies against p54 and p56. Whereas the approximately 4.5S class contains p42, p60, and p70, probably each in the form of individual molecules or small complexes, the approximately 6S particles appears to consist only of p54 and p56, which occur in a near-stoichiometric ratio suggestive of a heterodimer complex. The approximately 15S particles contain, in addition to p54 and p56, p60 and p100 and this is the single occurring form of RNA-binding p100. We have also observed changes in the in vitro mRNA binding properties of these polypeptides during oogenesis and early embryonic development, in relation to their phosphorylation state and to the activity of an approximately 15S particle-associated protein kinase, suggesting that these proteins are involved in the developmental translational regulation of maternal mRNAs.
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