ArticleNucleic acids research2006
Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein.
Article in Nucleic acids research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- USTAT: Unified Sequential Template Amplification and Transcription-a fully synthetic mRNA manufacturing platform.NPJ vaccines · 2026Article
- CLAE: A High-Fidelity Nanopore Sequencing Strategy for Read-Level Viral Variant Detection and Environmental RNA Virus Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.International journal of molecular sciences · 2023Review
- Review
- Single-stranded DNA aptamer-based rolling circle amplification as anti-chickenVeterinary world · 2022Article
- Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments.Biosensors · 2021Review
- Rolling circle amplification: A high fidelity and efficient alternative to plasmid preparation for the rescue of infectious clones.Virology · 2020Article
- CRISPR-Cas12a based internal negative control for nonspecific products of exponential rolling circle amplification.Nucleic acids research · 2020Article
- Highly sensitive and specific screening of EGFR mutation using a PNA microarray-based fluorometric assay based on rolling circle amplification and graphene oxide.RSC advances · 2019Article
- Resistance to UV Irradiation Caused by Inactivation ofJournal of bacteriology · 2018Article
- Are in vivo selections on the path to extinction?Microbial biotechnology · 2017Article
- DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication.Frontiers in molecular biosciences · 2016Review
- Helicase-Dependent Isothermal Amplification of DNA and RNA by Using Self-Avoiding Molecular Recognition Systems.Chembiochem : a European journal of chemical biology · 2015Article
- Rapid identification of black grain eumycetoma causative agents using rolling circle amplification.PLoS neglected tropical diseases · 2014Article
- Single molecule analysis of Thermus thermophilus SSB protein dynamics on single-stranded DNA.Nucleic acids research · 2014Article
- Massively parallel polymerase cloning and genome sequencing of single cells using nanoliter microwells.Nature biotechnology · 2013Article
- A mechanism for single-stranded DNA-binding protein (SSB) displacement from single-stranded DNA upon SSB-RecO interaction.The Journal of biological chemistry · 2011Article
- A non-canonical DNA structure enables homologous recombination in various genetic systems.The Journal of biological chemistry · 2009Article
- A procedure for highly specific, sensitive, and unbiased whole-genome amplification.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Genomic sequencing of single microbial cells from environmental samples.Current opinion in microbiology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rolling circle amplification (RCA) of plasmid or genomic DNA using random hexamers and bacteriophage phi29 DNA polymerase has become increasingly popular in the amplification of template DNA in DNA sequencing. We have found that the mutant protein of single-stranded DNA binding protein (SSB) from Thermus thermophilus (Tth) HB8 enhances the efficiency of amplification of DNA templates. In addition, the TthSSB mutant protein increased the specificity of phi29 DNA polymerase. We have overexpressed the native and mutant forms of TthSSB protein in Escherichia coli and purified them to homogeneity. In vitro, these proteins were found to bind specifically to single-stranded DNA. Addition of TthSSB mutant protein to RCA halved the elongation time required for phi29 DNA polymerase to synthesize DNA fragments in RCA. Furthermore, the presence of the TthSSB mutant protein essentially eliminates nonspecific DNA products in RCA reactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.