ArticleMolecular biology of the cell2006
Three-dimensional culture regulates Raf-1 expression to modulate fibronectin matrix assembly.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo.International journal of molecular sciences · 2018Article
- Developmental stage-dependent effects of cardiac fibroblasts on function of stem cell-derived engineered cardiac tissues.Scientific reports · 2017Article
- Three-dimensional culture systems in cancer research: Focus on tumor spheroid model.Pharmacology & therapeutics · 2016Review
- Cooperative effects of fibronectin matrix assembly and initial cell-substrate adhesion strength in cellular self-assembly.Acta biomaterialia · 2016Article
- p70 S6-kinase mediates the cooperation between Akt1 and Mek1 pathways in fibroblast-mediated extracellular matrix remodeling.Biochimica et biophysica acta · 2015Article
- The fate of internalized α5 integrin is regulated by matrix-capable fibronectin.The Journal of surgical research · 2014Article
- Study on the potential of RGD- and PHSRN-modified alginates as artificial extracellular matrices for engineering bone.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2013Article
- Direct and indirect control of mitogen-activated protein kinase pathway-associated components, BRAP/IMP E3 ubiquitin ligase and CRAF/RAF1 kinase, by the deubiquitylating enzyme USP15.The Journal of biological chemistry · 2012Article
- Article
- Lung self-assembly is modulated by tissue surface tensions.American journal of respiratory cell and molecular biology · 2011Article
- Measurement of aggregate cohesion by tissue surface tensiometry.Journal of visualized experiments : JoVE · 2011Article
- Article
- Alpha5beta1 integrin-fibronectin interactions specify liquid to solid phase transition of 3D cellular aggregates.PloS one · 2010Article
- Spheroid-based drug screen: considerations and practical approach.Nature protocols · 2009Article
- Ovarian normal and tumor-associated fibroblasts retain in vivo stromal characteristics in a 3-D matrix-dependent manner.Gynecologic oncology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Oncogenic transformation has been associated with decreased fibronectin (FN) matrix assembly. For example, both the HT-1080 fibrosarcoma and MAT-LyLu cell lines fail to assemble a FN matrix when grown in monolayer culture (2-dimensional [2D] system). In this study, we show that these cells regain the ability to assemble a FN matrix when they are grown as aggregates (3-dimensional [3D] system). FN matrix assembly in 3D correlates with decreased Raf-1 protein expression compared with cells grown in monolayer culture. This effect is associated with reduced Raf-1 mRNA levels as determined by quantitative RT-PCR and not proteasome-mediated degradation of endogenous Raf-1. Interestingly, transient expression of a Raf-1 promoter-reporter construct demonstrates increased Raf-1 promoter activity in 3D, suggesting that the transition to 3D culture may modulate Raf-1 mRNA stability. Finally, to confirm that decreased Raf-1 expression results in increased FN matrix assembly, we used both pharmacological and small interfering RNA knockdown of Raf-1. This restored the ability of cells in 2D culture to assemble a FN matrix. Moreover, overexpression of Raf-1 prevented FN matrix assembly by cells cultured in 3D, resulting in decreased aggregate compaction. This work provides new insight into how the cell microenvironment may influence Raf-1 expression to modulate cell-FN interactions in 3D.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.