ArticleMolecular biology of the cell2006
Targeting of transmembrane protein shrew-1 to adherens junctions is controlled by cytoplasmic sorting motifs.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Monoallelic de novoScience advances · 2024Article
- Tannic acid attenuates vascular calcification-induced proximal tubular cells damage through paracrine signaling.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Article
- AJAP1 expression modulates glioma cell motility and correlates with tumor growth and survival.International journal of oncology · 2018Article
- Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells.Biology open · 2017Article
- Alternative exon usage creates novel transcript variants of tumor suppressor SHREW-1 gene with differential tissue expression profile.Biology open · 2016Article
- Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma.Molecular cancer research : MCR · 2012Article
- A novel conserved targeting motif found in ABCA transporters mediates trafficking to early post-Golgi compartments.Journal of lipid research · 2011Article
- E-cadherin surface levels in epithelial growth factor-stimulated cells depend on adherens junction protein shrew-1.Molecular biology of the cell · 2009Article
- Faciogenital dysplasia protein (FGD1) regulates export of cargo proteins from the golgi complex via Cdc42 activation.Molecular biology of the cell · 2009Article
- Domain organization of long signal peptides of single-pass integral membrane proteins reveals multiple functional capacity.PloS one · 2008Article
- Morphogenesis of post-Golgi transport carriers.Histochemistry and cell biology · 2008Review
- Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells.Molecular biology of the cell · 2007Article
- Junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147.Molecular biology of the cell · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently identified transmembrane protein shrew-1 and showed that it is able to target to adherens junctions in polarized epithelial cells. This suggested shrew-1 possesses specific basolateral sorting motifs, which we analyzed by mutational analysis. Systematic mutation of amino acids in putative sorting signals in the cytoplasmic domain of shrew-1 revealed three tyrosines and a dileucine motif necessary for basolateral sorting. Substitution of these amino acids leads to apical localization of shrew-1. By applying tannic acid to either the apical or basolateral part of polarized epithelial cells, thereby blocking vesicle fusion with the plasma membrane, we obtained evidence that the apically localized mutants were primarily targeted to the basolateral membrane and were then redistributed to the apical domain. Further support for a postendocytic sorting mechanism of shrew-1 was obtained by demonstrating that mu1B, a subunit of the epithelial cell-specific adaptor complex AP-1B, interacts with shrew-1. In conclusion, our data provide evidence for a scenario where shrew-1 is primarily delivered to the basolateral membrane by a so far unknown mechanism. Once there, adaptor protein complex AP-1B is involved in retaining shrew-1 at the basolateral membrane by postendocytic sorting mechanisms.
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