ArticleThe Biochemical journal2006
Lipid raft components cholesterol and sphingomyelin increase H+/OH- permeability of phosphatidylcholine membranes.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Lumen alkalization under elevated bicarbonate concentration outside isolated thylakoids of higher plants.Photosynthesis research · 2026Article
- Unveiling the Future: Opportunities in Long-Acting Injectable Drug Development for Veterinary Care.Pharmaceutics · 2025Review
- Role of the Rumen Epithelium and Associated Changes Under High-Concentrate Diets.International journal of molecular sciences · 2025Review
- Activity Control of a Synthetic Transporter by Photodynamic Modulation of Membrane Mobility and Incorporation.Journal of the American Chemical Society · 2024Article
- Cryo-EM elucidates the uroplakin complex structure within liquid-crystalline lipids in the porcine urothelial membrane.Communications biology · 2023Article
- Antioxidant Interactions between Citrus Fruit Carotenoids and Ascorbic Acid in New Models of Animal Cell Membranes.Antioxidants (Basel, Switzerland) · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.bioRxiv : the preprint server for biology · 2023Article
- Fatty Acid-Activated Proton Transport by Bisaryl Anion Transporters Depolarises Mitochondria and Reduces the Viability of MDA-MB-231 Breast Cancer Cells.Biomolecules · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.Research square · 2023Article
- A Comprehensive Review on Novel Liposomal Methodologies, Commercial Formulations, Clinical Trials and Patents.BioNanoScience · 2022Review
- Sterol Extraction from Isolated Plant Plasma Membrane Vesicles Affects HBiomolecules · 2021Article
- Characterisation of a synthetic Archeal membrane reveals a possible new adaptation route to extreme conditions.Communications biology · 2021Article
- Perfusion Chamber for Observing a Liposome-Based Cell Model Prepared by a Water-in-Oil Emulsion Transfer Method.ACS omega · 2020Article
- Constructing artificial respiratory chain in polymer compartments: Insights into the interplay betweenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- NHE- and diffusion-dependent proton fluxes across the tubular system membranes of fast-twitch muscle fibers of the rat.The Journal of general physiology · 2018Article
- Ultrafast proton transport in sub-1-nm diameter carbon nanotube porins.Nature nanotechnology · 2016Article
- Effects of Lipid Tethering in Extremophile-Inspired Membranes on H(+)/OH(-) Flux at Room Temperature.Biophysical journal · 2016Article
- Osmotic homeostasis.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- The water permeability of lens aquaporin-0 depends on its lipid bilayer environment.Experimental eye research · 2013Article
- Water permeability of aquaporin-4 channel depends on bilayer composition, thickness, and elasticity.Biophysical journal · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
H+/OH- permeation through lipid bilayers occurs at anomalously high rates and the determinants of proton flux through membranes are poorly understood. Since all life depends on proton gradients, it is important to develop a greater understanding of proton leak phenomena. We have used stopped-flow fluorimetry to probe the influence of two lipid raft components, chol (cholesterol) and SM (sphingomyelin), on H+/OH- and water permeability. Increasing the concentrations of both lipids in POPC (palmitoyl-2-oleoyl phosphatidylcholine) liposomes decreased water permeability in a concentration-dependent manner, an effect that correlated with increased lipid order. Surprisingly, proton flux was increased by increasing the concentration of chol and SM. The chol effect was complex with molar concentrations of 17.9, 33 and 45.7% giving 2.8-fold (P<0.01), 2.2-fold (P<0.001) and 5.1-fold (P<0.001) increases in H+/OH- permeability from a baseline of 2.4x10(-2) cm/s. SM at 10 mole% effected a 2.8-fold increase (P<0.01), whereas 20 and 30 mole% enhanced permeability by 3.6-fold (P<0.05) and 4.1-fold respectively (P<0.05). Supplementing membranes containing chol with SM did not enhance H+/OH- permeability. Of interest was the finding that chol addition to soya-bean lipids decreased H+/OH- permeability, consistent with an earlier report [Ira and Krishnamoorthy (2001) J. Phys. Chem. B 105, 1484-1488]. We speculate that the presence of proton carriers in crude lipid extracts might contribute to this result. We conclude that (i) chol and SM specifically and independently increase rates of proton permeation in POPC bilayers, (ii) domains enriched in these lipids or domain interfaces may represent regions with high H+/OH- conductivity, (iii) H+/OH- fluxes are not governed by lipid order and (iv) chol can inhibit or promote H+/OH- permeability depending on the total lipid environment. Theories of proton permeation are discussed in the light of these results.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.