ArticleMolecular genetics and genomics : MGG2006
AFLP and PCR-based markers linked to Rf3, a fertility restorer gene for S cytoplasmic male sterility in maize.
Article in Molecular genetics and genomics : MGG, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Cytoplasmic genome contributions to domestication and improvement of modern maize.BMC biology · 2024Article
- Identification and validation of genetic locus Rfk1 for wheat fertility restoration in the presence of Aegilops kotschyi cytoplasm.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
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- Cytoplasmic male sterility (CMS) in hybrid breeding in field crops.Plant cell reports · 2016Review
- Multiple loci not only Rf3 involved in the restoration ability of pollen fertility, anther exsertion and pollen shedding to S type cytoplasmic male sterile in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Genomics for greater efficiency in pigeonpea hybrid breeding.Frontiers in plant science · 2015Review
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Authors and funding
3 authors.
Funding
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Abstract
The Rf3 gene restores the pollen fertility disturbed by S male sterile cytoplasm. In order to develop molecular markers tightly linked to Rf3, we used amplified fragment length polymorphism (AFLP) technique with near isogenic lines (NILs) and bulk segregant analysis (BSA). A BC(1)F(1) population from a pair of NILs with different Rf3 locus was constructed and 528 primer combinations was screened. A linkage map was constructed around the Rf3 locus, which was mapped on the distal region of chromosome 2 long arm with the help of SSR marker UMC2184. The closest marker E7P6 was 0.9 cM away from Rf3. Marker E3P1, 2.4 cM from Rf3, and E12M7, 1.8 cM from Rf3, were converted into a codominant CAPS and a dominant SCAR marker, and designated as CAPSE3P1 and SCARE12M7, respectively. These markers are useful for marker-assisted selection and map-based cloning of the Rf3 gene.
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