ArticleMolecular and cellular biology2006
Phosphorylation of Jak2 on Ser(523) inhibits Jak2-dependent leptin receptor signaling.
Article in Molecular and cellular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Review
- Proteomic and metabolomic analysis of GH deficiency-induced NAFLD in hypopituitarism: insights into oxidative stress.Frontiers in endocrinology · 2024Article
- Insights into the Potential Mechanisms of JAK2V617F Somatic Mutation Contributing Distinct Phenotypes in Myeloproliferative Neoplasms.International journal of molecular sciences · 2022Review
- JAK2S523L, a novel gain-of-function mutation in a critical autoregulatory residue in JAK2V617F- MPNs.Blood advances · 2020Article
- Mechanistic Insights into Regulation of JAK2 Tyrosine Kinase.Frontiers in endocrinology · 2017Review
- Diet-induced obese mice retain endogenous leptin action.Cell metabolism · 2015Article
- Review
- The molecular regulation of Janus kinase (JAK) activation.The Biochemical journal · 2014Review
- Molecular basis for pseudokinase-dependent autoinhibition of JAK2 tyrosine kinase.Nature structural & molecular biology · 2014Article
- JAK/STAT signaling in hematological malignancies.Oncogene · 2013Review
- Enu mutagenesis identifies a novel platelet phenotype in a loss-of-function Jak2 allele.PloS one · 2013Article
- Crystal structures of the JAK2 pseudokinase domain and the pathogenic mutant V617F.Nature structural & molecular biology · 2012Article
- Biology and significance of the JAK/STAT signalling pathways.Growth factors (Chur, Switzerland) · 2012Review
- Janus kinase 3: the controller and the controlled.Acta biochimica et biophysica Sinica · 2012Review
- JAK Kinases in Health and Disease: An Update.The open rheumatology journal · 2012Article
- Adiponectin inhibits leptin signalling via multiple mechanisms to exert protective effects against hepatic fibrosis.The Biochemical journal · 2011Article
- The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling.Nature structural & molecular biology · 2011Article
- Analysis of Jak2 catalytic function by peptide microarrays: the role of the JH2 domain and V617F mutation.PloS one · 2011Article
- Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle.American journal of physiology. Cell physiology · 2010Article
- A regulating role of the JAK2 FERM domain in hyperactivation of JAK2(V617F).The Biochemical journal · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
The leptin receptor, LRb, and other cytokine receptors are devoid of intrinsic enzymatic activity and rely upon the activity of constitutively associated Jak family tyrosine kinases to mediate intracellular signaling. In order to clarify mechanisms by which Jak2, the cognate LRb-associated Jak kinase, is regulated and mediates downstream signaling, we employed tandem mass spectroscopic analysis to identify phosphorylation sites on Jak2. We identified Ser523 as the first-described site of Jak2 serine phosphorylation and demonstrated that this site is phosphorylated on Jak2 from intact cells and mouse spleen. Ser523 was highly phosphorylated in HEK293 cells independently of LRb-Jak2 activation, suggesting a potential role for the phosphorylation of Ser523 in the regulation of LRb by other pathways. Indeed, mutation of Ser523 sensitized and prolonged signaling by Jak2 following activation by the intracellular domain of LRb. The effect of Ser523 on Jak2 function was independent of Tyr570-mediated inhibition. Thus, the phosphorylation of Jak2 on Ser523 inhibits Jak2 activity and represents a novel mechanism for the regulation of Jak2-dependent cytokine signaling.
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