ArticleArthritis research & therapy2006
Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice.
Article in Arthritis research & therapy, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- HTRA1-driven detachment of type I collagen from endoplasmic reticulum contributes to myocardial fibrosis in dilated cardiomyopathy.Journal of translational medicine · 2024Article
- Cholesterol determines the cytosolic entry and seeded aggregation of tau.Cell reports · 2022Article
- Uncovering mediators of collagen degradation in the tumor microenvironment.Matrix biology plus · 2022Article
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- Collagen Biosynthesis, Processing, and Maturation in Lung Ageing.Frontiers in medicine · 2021Review
- Review
- Macropinocytosis: New Insights Into Its Underappreciated Role in Innate Immune Cell Surveillance.Frontiers in immunology · 2018Review
- Cytokine Imbalance as a Common Mechanism in Both Psoriasis and Rheumatoid Arthritis.Mediators of inflammation · 2017Article
- Calcium-sensing receptors signal constitutive macropinocytosis and facilitate the uptake of NOD2 ligands in macrophages.Nature communications · 2016Article
- Macropinocytosis in phagocytes: regulation of MHC class-II-restricted antigen presentation in dendritic cells.Frontiers in physiology · 2015Review
- Physiological role of the interaction between CARMIL1 and capping protein.Molecular biology of the cell · 2013Article
- Always cleave up your mess: targeting collagen degradation to treat tissue fibrosis.American journal of physiology. Lung cellular and molecular physiology · 2013Review
- Pathways of antigen processing.Annual review of immunology · 2013Review
- SNX5 is essential for efficient macropinocytosis and antigen processing in primary macrophages.Biology open · 2012Article
- Article
- Aspect ratio determines the quantity of mesoporous silica nanoparticle uptake by a small GTPase-dependent macropinocytosis mechanism.ACS nano · 2011Article
- A differential role for macropinocytosis in mediating entry of the two forms of vaccinia virus into dendritic cells.PLoS pathogens · 2010Article
- Endocytosis and intracellular trafficking of human natural killer cell receptors.Traffic (Copenhagen, Denmark) · 2009Review
- LPS activation is required for migratory activity and antigen presentation by tolerogenic dendritic cells.Journal of leukocyte biology · 2009Article
- Endocytosis as a mechanism of regulating natural killer cell function: unique endocytic and trafficking pathway for CD94/NKG2A.Immunologic research · 2009Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Professional antigen-presenting cells, such as dendritic cells, macrophages and B cells have been implicated in the pathogenesis of rheumatoid arthritis, constituting a possible target for antigen-specific immunotherapy. We addressed the possibility of blocking antigen presentation of the type II collagen (CII)-derived immunodominant arthritogenic epitope CII259-273 to specific CD4 T cells by inhibition of antigen uptake in HLA-DR1-transgenic mice in vitro and in vivo. Electron microscopy, confocal microscopy, subcellular fractionation and antigen presentation assays were used to establish the mechanisms of uptake, intracellular localization and antigen presentation of CII by dendritic cells and macrophages. We show that CII accumulated in membrane fractions of intermediate density corresponding to late endosomes. Treatment of dendritic cells and macrophages with cytochalasin D or amiloride prevented the intracellular appearance of CII and blocked antigen presentation of CII259-273 to HLA-DR1-restricted T cell hybridomas. The data suggest that CII was taken up by dendritic cells and macrophages predominantly via macropinocytosis. Administration of amiloride in vivo prevented activation of CII-specific polyclonal T cells in the draining popliteal lymph nodes. This study suggests that selective targeting of CII internalization in professional antigen-presenting cells prevents activation of autoimmune T cells, constituting a novel therapeutic strategy for the immunotherapy of rheumatoid arthritis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.