ArticleProceedings of the National Academy of Sciences of the United States of America2006
The TodS-TodT two-component regulatory system recognizes a wide range of effectors and works with DNA-bending proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 82 citations in OpenAlex.
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- Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.Environmental microbiology · 2022Article
- The pH Robustness of Bacterial Sensing.mBio · 2022Article
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- Transcriptional Regulation of the Peripheral Pathway for the Anaerobic Catabolism of Toluene andFrontiers in microbiology · 2018Article
- Metabolic Value Chemoattractants Are Preferentially Recognized at Broad Ligand Range Chemoreceptor ofFrontiers in microbiology · 2017Article
- New family of biosensors for monitoring BTX in aquatic and edaphic environments.Microbial biotechnology · 2016Article
- Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.The Journal of biological chemistry · 2016Article
- Multiple signals modulate the activity of the complex sensor kinase TodS.Microbial biotechnology · 2015Article
- A tale of two machines: a review of the BLAST meeting, Tucson, AZ, 20-24 January 2013.Molecular microbiology · 2014Review
- IHF is required for the transcriptional regulation of the Desulfovibrio vulgaris Hildenborough orp operons.PloS one · 2014Article
- A MarR-Type regulator directly activates transcription from the Brucella abortus virB promoter by sharing a redundant role with HutC.Journal of bacteriology · 2012Article
- Construction and application of an Escherichia coli bioreporter for aniline and chloroaniline detection.Journal of industrial microbiology & biotechnology · 2012Article
- Study of the TmoS/TmoT two-component system: towards the functional characterization of the family of TodS/TodT like systems.Microbial biotechnology · 2012Article
- Dual two-component regulatory systems are involved in aromatic compound degradation in a polychlorinated-biphenyl degrader, Rhodococcus jostii RHA1.Journal of bacteriology · 2010Article
- Catabolite repression of the TodS/TodT two-component system and effector-dependent transphosphorylation of TodT as the basis for toluene dioxygenase catabolic pathway control.Journal of bacteriology · 2010Article
- Reconstructing the evolutionary history of nitrotoluene detection in the transcriptional regulator NtdR.Molecular microbiology · 2009Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The TodS and TodT proteins form a previously unrecognized and highly specific two-component regulatory system in which the TodS sensor protein contains two input domains, each of which are coupled to a histidine kinase domain. This system regulates the expression of the genes involved in the degradation of toluene, benzene, and ethylbenzene through the toluene dioxygenase pathway. In contrast to the narrow substrate range of this catabolic pathway, the TodS effector profile is broad. TodS has basal autophosphorylation activity in vitro, which is enhanced by the presence of effectors. Toluene binds to TodS with high affinity (Kd = 684 +/- 13 nM) and 1:1 stoichiometry. The analysis of the truncated variants of TodS reveals that toluene binds to the N-terminal input domain (Kd = 2.3 +/- 0.1 microM) but not to the C-terminal half. TodS transphosphorylates TodT, which binds to two highly similar DNA binding sites at base pairs -107 and -85 of the promoter. Integration host factor (IHF) plays a crucial role in the activation process and binds between the upstream TodT boxes and the -10 hexamer region. In an IHF-deficient background, expression from the tod promoter drops 8-fold. In vitro transcription assays confirmed the role determined in vivo for TodS, TodT, and IHF. A functional model is presented in which IHF favors the contact between the TodT activator, bound further upstream, and the alpha-subunit of RNA polymerase bound to the downstream promoter element. Once these contacts are established, the tod operon is efficiently transcribed.
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