ArticleGenetics2006
Transcriptionally silenced transgenes in maize are activated by three mutations defective in paramutation.
Article in Genetics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 39 citations in OpenAlex.
- Comprehensive Mechanism of Gene Silencing and Its Role in Plant Growth and Development.Frontiers in plant science · 2021Review
- Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.Frontiers in plant science · 2019Article
- Subtle Perturbations of the Maize Methylome Reveal Genes and Transposons Silenced by Chromomethylase or RNA-Directed DNA Methylation Pathways.G3 (Bethesda, Md.) · 2018Article
- Altered nucleosome positions in maize haplotypes and mutants of a subset of SWI/SNF-like proteins.Plant direct · 2017Article
- Article
- Exploring the secrets of long noncoding RNAs.International journal of molecular sciences · 2015Review
- Epigenetic silencing in transgenic plants.Frontiers in plant science · 2015Review
- Genetic perturbation of the maize methylome.The Plant cell · 2014Article
- Diverse gene-silencing mechanisms with distinct requirements for RNA polymerase subunits in Zea mays.Genetics · 2014Article
- Chromatin structure and gene expression changes associated with loss of MOP1 activity in Zea mays.Epigenetics · 2014Article
- Paramutagenicity of a p1 epiallele in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Rapid divergence of prolamin gene promoters of maize after gene amplification and dispersal.Genetics · 2012Article
- required to maintain repression2 is a novel protein that facilitates locus-specific paramutation in maize.The Plant cell · 2012Article
- Computational identification and functional predictions of long noncoding RNA in Zea mays.PloS one · 2012Article
- Identification and characterization of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families in maize.Plant cell reports · 2011Article
- Identification of epigenetic regulators of a transcriptionally silenced transgene in maize.G3 (Bethesda, Md.) · 2011Article
- RNA-mediated trans-communication can establish paramutation at the b1 locus in maize.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- A dominant mutation in mediator of paramutation2, one of three second-largest subunits of a plant-specific RNA polymerase, disrupts multiple siRNA silencing processes.PLoS genetics · 2009Article
- Article
- Distinct size distribution of endogeneous siRNAs in maize: Evidence from deep sequencing in the mop1-1 mutant.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants with mutations in one of three maize genes, mop1, rmr1, and rmr2, are defective in paramutation, an allele-specific interaction that leads to meiotically heritable chromatin changes. Experiments reported here demonstrate that these genes are required to maintain the transcriptional silencing of two different transgenes, suggesting that paramutation and transcriptional silencing of transgenes share mechanisms. We hypothesize that the transgenes are silenced through an RNA-directed chromatin mechanism, because mop1 encodes an RNA-dependent RNA polymerase. In all the mutants, DNA methylation was reduced in the active transgenes relative to the silent transgenes at all of the CNG sites monitored within the transgene promoter. However, asymmetrical methylation persisted at one site within the reactivated transgene in the rmr1-1 mutant. With that one mutant, rmr1-1, the transgene was efficiently resilenced upon outcrossing to reintroduce the wild-type protein. In contrast, with the mop1-1 and rmr2-1 mutants, the transgene remained active in a subset of progeny even after the wild-type proteins were reintroduced by outcrossing. Interestingly, this immunity to silencing increased as the generations progressed, consistent with a heritable chromatin state being formed at the transgene in plants carrying the mop1-1 and rmr2-1 mutations that becomes more resistant to silencing in subsequent generations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.