ArticleActa biomaterialia2005
Substrate-mediated delivery from self-assembled monolayers: effect of surface ionization, hydrophilicity, and patterning.
Article in Acta biomaterialia, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Bio-inspired polyphenol-polyelectrolyte WRAP system for geometrically controlled AAV delivery in diverse anatomical sites.Materials today. Bio · 2026Article
- DNA Nanostructure Deposition on Self-Assembled Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Adeno-Associated Viral Vector Immobilization and Local Delivery from Bare Metal Surfaces.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Carbon Nano-Onions Reinforced Multilayered Thin Film System for Stimuli-Responsive Drug Release.Pharmaceutics · 2020Article
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery on Titanium Substrates Modified With RGD-Functionalized Poly(acrylic acid) Brushes.Frontiers in chemistry · 2019Article
- Layer-by-layer assembled polymeric thin films as prospective drug delivery carriers: design and applications.Biomaterials research · 2018Review
- MicroRNA delivery for regenerative medicine.Advanced drug delivery reviews · 2015Review
- The role of surface chemistry-induced cell characteristics on nonviral gene delivery to mouse fibroblasts.Journal of biological engineering · 2012Article
- Rapid release of plasmid DNA from surfaces coated with polyelectrolyte multilayers promoted by the application of electrochemical potentials.ACS applied materials & interfaces · 2012Article
- High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts.Biomaterials · 2011Article
- Surface immobilization of plasmid DNA with a cell-responsive tether for substrate-mediated gene delivery.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.Nano today · 2010Article
- Patterned transgene expression in multiple-channel bridges after spinal cord injury.Acta biomaterialia · 2010Article
- Lentivirus delivery by adsorption to tissue engineering scaffolds.Journal of biomedical materials research. Part A · 2010Article
- Phosphatidylserine immobilization of lentivirus for localized gene transfer.Biomaterials · 2010Article
- Engineering surfaces for substrate-mediated gene delivery using recombinant proteins.Biomacromolecules · 2009Article
- Fibrin hydrogels for non-viral vector delivery in vitro.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Multilayer mediated forward and patterned siRNA transfection using linear-PEI at extended N/P ratios.Acta biomaterialia · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Gene transfer has many potential applications in basic and applied sciences. In vitro, DNA delivery can be enhanced by increasing the concentration of DNA in the cellular microenvironment through immobilization of DNA to a substrate that supports cell adhesion. Substrate-mediated delivery describes the immobilization of DNA, complexed with cationic lipids or polymers, to a biomaterial or substrate. As surface properties are critical to the efficiency of the surface delivery approach, self-assembled monolayers (SAMs) of alkanethiols on gold were used to correlate surface chemistry of the substrate to binding, release, and transfection of non-specifically immobilized complexes. Surface hydrophobicity and ionization were found to mediate both DNA complex immobilization and transfection, but had no effect on complex release. Additionally, SAMs were used in conjunction with soft lithographic techniques to imprint substrates with specific patterns, resulting in patterned DNA complex deposition and transfection, with transfection efficiencies in the patterns nearing 40%. Controlling the interactions between complexes and substrates, with the potential for patterned delivery, can be used to locally enhance or regulate gene transfer, with applications to tissue engineering scaffolds and transfected cell arrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.