Evidence map›Paper›PMID 16701558›Full record

ArticleBiochemical and biophysical research communications2006

Ceramide response post-photodamage is absent after treatment with HA14-1.

Duska Separovic, Shouye Wang, Ma'In Yehya Awad Maitah, Kentaro Hanada, David Kessel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemical and biophysical research communications, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Duska SeparovicDepartment of Fundamental and Applied Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48201, USA. dseparovic@wayne.edu
Shouye Wang
Ma'In Yehya Awad Maitah
Kentaro Hanada
David Kessel
Eugene Applebaum College of Pharmacy and Health Sciences · USNational Institute of Infectious Diseases · JPWayne State University · US

Funding

Subcellular targets and PDT-induces cell deathR01CA023378 · NCI · WAYNE STATE UNIVERSITY · PI KESSEL, DAVID HARRY · 1985 to 2013
$3.3M
Sphingolipids in Cell Death after PhotodamageR01CA077475 · NCI · WAYNE STATE UNIVERSITY · PI SEPAROVIC, DUSKA M · 2005 to 2012
$1.6M
NCI NIH HHS CA23378NCI NIH HHS R01 CA023378NCI NIH HHS R01 CA077475NCI NIH HHS R01 CA77475
6 · The paper itself

Abstract

The oxidative stress induced by photodynamic therapy using the phthalocyanine Pc 4 (PDT) can lead to apoptosis, and is accompanied by photodamage to Bcl-2 and accumulation of de novo ceramide. Similar to PDT, the oxidative stress inducer and Bcl-2 inhibitor HA14-1 triggers apoptosis. To test the specificity of the ceramide response, Jurkat cells were exposed to an equitoxic dose of HA14-1. Unlike PDT, HA14-1 did not induce accumulation of de novo ceramide, although levels of sphingomyelin, phosphatidylserine and phosphatidylethanolamine were below control values after either treatment. In contrast to PDT, (i) the transient inhibition of serine palmitoyltransferase induced by HA14-1 was associated with the initial decrease in de novo ceramide, and (ii) HA14-1-initiated inhibition of sphingomyelin synthase and glucosylceramide synthase did not result in accumulation of de novo ceramide. These results show that the ceramide response to PDT is not induced by another pro-apoptotic stimulus, and may be unique to PDT as described here.

Indexed as

ApoptosisBenzopyransCells, CulturedCeramidesGlucosyltransferasesHumansJurkat CellsNitrilesPhosphatidylethanolaminesPhosphatidylserinesPhotochemotherapySerine C-PalmitoyltransferaseSphingomyelinsTime FactorsTransferases (Other Substituted Phosphate Groups)Benzopyransceramide glucosyltransferaseCeramidesethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylateGlucosyltransferasesNitrilesphosphatidylcholine-ceramide phosphocholine transferasephosphatidylethanolaminePhosphatidylethanolaminesPhosphatidylserinesSerine C-PalmitoyltransferaseSphingomyelinsTransferases (Other Substituted Phosphate Groups)

Identifiers

PMID16701558
PMCPMC2972543
OpenAlexW2038534208

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.