ArticleRNA (New York, N.Y.)2006
Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair.Journal of nanobiotechnology · 2024Review
- PepFect14 mediates the delivery of mRNA into human primary keratinocytes andFrontiers in pharmacology · 2023Article
- Transferrin receptor targeting segment T7 containing peptide gene delivery vectors for efficient transfection of brain tumor cells.Drug delivery · 2022Article
- Cell-Penetrating Peptides Delivering siRNAs: An Overview.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Article
- Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers.Life (Basel, Switzerland) · 2019Review
- Efficient siRNA delivery and gene silencing using a lipopolypeptide hybrid vector mediated by a caveolae-mediated and temperature-dependent endocytic pathway.Journal of nanobiotechnology · 2019Article
- The structure and configuration changes of multifunctional peptide vectors enhance gene delivery efficiency.RSC advances · 2018Article
- Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain.Cell death & disease · 2017Article
- Functional peptides for siRNA delivery.Advanced drug delivery reviews · 2017Review
- Bone scaffolds loaded with siRNA-Semaphorin4d for the treatment of osteoporosis related bone defects.Scientific reports · 2016Article
- Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides.Nucleic acid therapeutics · 2015Article
- Attachment of cell-binding ligands to arginine-rich cell-penetrating peptides enables cytosolic translocation of complexed siRNA.Chemistry & biology · 2015Article
- Highly efficient method for gene delivery into mouse dorsal root ganglia neurons.Frontiers in molecular neuroscience · 2015Article
- Effective small interfering RNA delivery in vitro via a new stearylated cationic peptide.International journal of nanomedicine · 2015Article
- Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA.International journal of nanomedicine · 2015Article
- Prevention of alveolar bone loss in an osteoporotic animal model via interference of semaphorin 4d.Journal of dental research · 2014Article
- Preconditioning stimuli induce autophagy via sphingosine kinase 2 in mouse cortical neurons.The Journal of biological chemistry · 2014Article
- Development of cholesteryl peptide micelles for siRNA delivery.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- In vitro investigations of the efficacy of cyclodextrin-siRNA complexes modified with lipid-PEG-Octaarginine: towards a formulation strategy for non-viral neuronal siRNA delivery.Pharmaceutical research · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) provides a powerful experimental tool for sequence-specific gene silencing, allowing efficient analysis of gene function in a multitude of cell types. However, application of RNAi in primary mammalian neurons has been limited by low-transfection efficiency and considerable toxicity of conventional transfection methods. In this study, we evaluated a peptide-mediated and a polymer/lipid-based cellular delivery method for siRNA into rat primary neurons and compared the results with a commonly used liposomal transfection reagent. Stearylated octaarginine (Stearyl-R8) was used as polypeptide and artificial virus-like particles (AVPs) were used as a combined liposomal-polymeric vector, since both reagents have been previously shown to successfully transfect DNA into cell lines. Stearyl-R8 and AVPs both promoted siRNA transfection into primary hippocampal neurons via the endosomal pathway. SiRNA-mediated gene silencing could be effectively induced in primary neuron cultures. In comparison with the commonly used cationic liposome transfection agent, both novel reagents were less detrimental to cell metabolic activity. We conclude that these novel transfection methods yield performances comparable to cationic liposome-mediated transfection for siRNA, while being less cytotoxic in primary neurons. Stearyl-R8 and AVPs may therefore represent novel and more cost-efficient alternatives to conventional siRNA-transfection reagents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.