ArticlePlant physiology2006
The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis.
Article in Plant physiology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed, 153 citations in OpenAlex.
- Turning over a green leaf: integrating light signals toward chloroplast establishment.Plant physiology · 2026Review
- Membranous Interacting Partners of Phage-Type Plastid RNA Polymerase Have Limited Impact on Plastid Gene Expression During Chloroplast Development.Plant direct · 2026Article
- Apocarotenoid signaling regulates meristem activity and shapes shoot and root lateral organ formation in Arabidopsis.Plant physiology · 2025Article
- Light and Shadows: Insights from Large-Scale Visual Screens for Arabidopsis Leaf Morphology Mutants.International journal of molecular sciences · 2025Review
- RNA Polymerase RPOTp is Involved in C-to-U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Chloroplasts prevent precocious flowering through a GOLDEN2-LIKE-B-BOX DOMAIN PROTEIN module.Plant communications · 2023Article
- Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants.Plant communications · 2023Article
- Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes.Nature communications · 2022Article
- A Transcriptomic Analysis of Tobacco Leaf with the Functional Loss of the PlastidPlants (Basel, Switzerland) · 2022Article
- Complete Chloroplast Genome ofGenes · 2022Article
- H4 acetylation by the NuA4 complex is required for plastid transcription and chloroplast biogenesis.Nature plants · 2022Article
- Missplicing suppressor alleles of Arabidopsis PRE-MRNA PROCESSING FACTOR 8 increase splicing fidelity by reducing the use of novel splice sites.Nucleic acids research · 2022Article
- Chlorosis seedling lethality 1 encoding a MAP3K protein is essential for chloroplast development in rice.BMC plant biology · 2022Article
- Riddled with holes: Understanding air space formation in plant leaves.PLoS biology · 2021Review
- Article
- A Transcription Factor Regulates Gene Expression in Chloroplasts.International journal of molecular sciences · 2021Article
- GUN control in retrograde signaling: How GENOMES UNCOUPLED proteins adjust nuclear gene expression to plastid biogenesis.The Plant cell · 2021Review
- A pentatricopeptide repeat protein DUA1 interacts with sigma factor 1 to regulate chloroplast gene expression in Rice.Photosynthesis research · 2021Article
- Changes in plastid biogenesis leading to the formation of albino regenerants in barley microspore culture.BMC plant biology · 2021Article
- Albino Plant Formation in Androgenic Cultures: An Old Problem and New Facts.Methods in molecular biology (Clifton, N.J.) · 2021Review
10 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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Abstract
In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and two strong (sca3-2 and sca3-3) alleles of the SCA3 gene, the latter two showing severely impaired plant growth and reduced pigmentation of the cotyledons, leaves, stem, and sepals, all of which were pale green. The leaf surface was extremely crumpled in the sca3 mutants, although epidermal cell size and morphology were not perturbed, whereas the mesophyll cells were less densely packed and more irregular in shape than in the wild type. A significant reduction in the size, morphology, and number of chloroplasts was observed in homozygous sca3-2 individuals whose photoautotrophic growth was consequently perturbed. Microarray analysis showed that several hundred nuclear genes were differentially expressed in sca3-2 and the wild type, about one-fourth of which encoded chloroplast-targeted proteins. Quantitative reverse transcription-PCR analyses showed that the sca3-2 mutation alters the expression of the rpoB, rpoC1, clpP, and accD plastid genes and the SCA3 paralogs RpoTm and RpoTmp, which respectively encode nuclear-encoded mitochondrion or dually targeted RNA polymerases. Double-mutant analysis indicated that RpoTmp and SCA3 play redundant functions in plant development. Our findings support a role for plastids in leaf morphogenesis and indicate that RpoTp is required for mesophyll cell proliferation.
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