ArticleBiophysical journal2006
Determination of the orientational distribution and orientation factor for transfer between membrane-bound fluorophores using a confocal microscope.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
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- Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration.Nature communications · 2022Article
- FRET-FLIM to Determine Protein Interactions and Membrane Topology of Enzyme Complexes.Current protocols · 2022Article
- Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2).Scientific reports · 2022Article
- Simple estimation of Förster Resonance Energy Transfer (FRET) orientation factor distribution in membranes.International journal of molecular sciences · 2012Article
- Probing the orientational distribution of dyes in membranes through multiphoton microscopy.Biophysical journal · 2012Article
- Förster resonance energy transfer as a probe of membrane protein folding.Biochimica et biophysica acta · 2012Article
- Förster resonance energy transfer (FRET) correlates of altered subunit stoichiometry in cys-loop receptors, exemplified by nicotinic α4β2.International journal of molecular sciences · 2012Article
- The T cell receptor triggering apparatus is composed of monovalent or monomeric proteins.The Journal of biological chemistry · 2011Article
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- An improved open-channel structure of MscL determined from FRET confocal microscopy and simulation.The Journal of general physiology · 2010Article
- Fluorescence lifetime measurements and biological imaging.Chemical reviews · 2010Review
- Structural changes in the cytoplasmic domain of the mechanosensitive channel MscS during opening.Biophysical journal · 2009Article
- Impact of emission anisotropy on fluorescence spectroscopy and FRET distance measurements.Biophysical journal · 2009Article
- Nicotine normalizes intracellular subunit stoichiometry of nicotinic receptors carrying mutations linked to autosomal dominant nocturnal frontal lobe epilepsy.Molecular pharmacology · 2009Article
- Probing complexes with single fluorophores: factors contributing to dispersion of FRET in DNA/RNA duplexes.European biophysics journal : EBJ · 2009Article
- Imaging the boundaries-innovative tools for microscopy of living cells and real-time imaging.Journal of chemical biology · 2008Article
- Simulation of structure, orientation, and energy transfer between AlexaFluor molecules attached to MscL.Biophysical journal · 2008Article
- Effect of membrane microheterogeneity and domain size on fluorescence resonance energy transfer.Biophysical journal · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Orientational fluorophores have been a useful tool in physical chemistry, biochemistry, and more recently structural biology due to the polarized nature of the light they emit and that fact that energy can be transferred between them. We present a practical scheme in which measurements of the intensity of emitted fluorescence can be used to determine limits on the mean and distribution of orientation of the absorption transition moment of membrane-bound fluorophores. We demonstrate how information about the orientation of fluorophores can be used to calculate the orientation factor kappa(2) required for use in FRET spectroscopy. We illustrate the method using images of AlexaFluor probes bound to MscL mechanosensitive transmembrane channel proteins in spherical liposomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.