ArticleDevelopmental biology2006
Fertilization triggers localized activation of Src-family protein kinases in the zebrafish egg.
Article in Developmental biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Knockin' on Egg's Door: Maternal Control of Egg Activation That Influences Cortical Granule Exocytosis in Animal Species.Frontiers in cell and developmental biology · 2021Review
- Genome-Wide Identification and Characterization of Tyrosine Kinases in the Silkworm, Bombyx mori.International journal of molecular sciences · 2018Article
- Phosphoinositide 5- and 3-phosphatase activities of a voltage-sensing phosphatase in living cells show identical voltage dependence.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- SRC-family tyrosine kinases in oogenesis, oocyte maturation and fertilization: an evolutionary perspective.Advances in experimental medicine and biology · 2014Review
- Conservation and early expression of zebrafish tyrosine kinases support the utility of zebrafish as a model for tyrosine kinase biology.Zebrafish · 2013Article
- Protein tyrosine kinase signaling in the mouse oocyte cortex during sperm-egg interactions and anaphase resumption.Molecular reproduction and development · 2013Article
- Intersecting roles of protein tyrosine kinase and calcium signaling during fertilization.Cell calcium · 2013Review
- PYK2: a calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte.Developmental biology · 2013Article
- Mass spectrometric analysis of mono- and multi-phosphopeptides by selective binding with NiZnFe₂O₄ magnetic nanoparticles.Nature communications · 2013Article
- Dynamics of PLCγ and Src family kinase 1 interactions during nuclear envelope formation revealed by FRET-FLIM.PloS one · 2012Article
- Fer tyrosine kinase is required for germinal vesicle breakdown and meiosis-I in mouse oocytes.Molecular reproduction and development · 2011Article
- Distribution of laminin β2, collagen type IV, fibronectin and MMP-9 in ovaries of the teleost fish.Journal of molecular histology · 2010Article
- Evidence that phosphatidylinositol 3-kinase is involved in sperm-induced tyrosine kinase signaling in Xenopus egg fertilization.BMC developmental biology · 2009Article
- Fyn kinase activity is required for normal organization and functional polarity of the mouse oocyte cortex.Molecular reproduction and development · 2009Article
- hnRNP I is required to generate the Ca2+ signal that causes egg activation in zebrafish.Development (Cambridge, England) · 2009Article
- Localized activation of Src-family protein kinases in the mouse egg.Developmental biology · 2007Article
- Protein tyrosine kinase signaling during oocyte maturation and fertilization.Molecular reproduction and developmentReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Fertilization triggers activation of Src-family kinases in eggs of various species including marine invertebrates and lower vertebrates. While immunofluorescence studies have localized Src-family kinases to the plasma membrane or cortical cytoplasm, no information is available regarding the extent to which these kinases are activated in different regions of the zygote. The objective of the present study was to detect the subcellular distribution of activated Src-family kinases in the fertilized zebrafish egg. An antibody specific for the active, non-phosphorylated form of Src-family PTKs was used to detect these activated kinases by immunofluorescence. The results demonstrate that Fyn, and possibly other Src family members are activated by dephosphorylation of the C-terminal tyrosine at fertilization. The activated Src-family kinases are asymmetrically distributed around the egg cortex with an area of higher kinase activity localized adjacent to the micropyle near the presumptive animal pole. Fertilization initially caused elevation of kinase activity in the cytoplasm underlying the micropyle, but this quickly spread to involve the entire zygote cortex. Later, during egg activation, formation of the blastodisc involved concentration of active Src-family kinase in the blastodisc cortex. As cytokinesis began, activated Src-family kinases were no longer limited to the cortex, but became more evenly distributed in the clear apical cytoplasm of the blastomeres. The results demonstrate that the cortex of the zebrafish egg is functionally differentiated and that fertilization triggers localized activation of Src-family kinases at the point of sperm entry, which subsequently progresses through the entire egg cortex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.