ArticleRNA (New York, N.Y.)2006
RBP16 stimulates trypanosome RNA editing in vitro at an early step in the editing reaction.
Article in RNA (New York, N.Y.), 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Sulfonated inhibitors of the RNA editing ligases validate the essential role of the MRP1/2 proteins in kinetoplastid RNA editing.RNA (New York, N.Y.) · 2020Article
- PRMT7 regulates RNA-binding capacity and protein stability in Leishmania parasites.Nucleic acids research · 2020Article
- Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.Trends in parasitology · 2020Review
- The RNA binding activity of the first identified trypanosome protein with Z-DNA-binding domains.Scientific reports · 2019Article
- Combinatorial interplay of RNA-binding proteins tunes levels of mitochondrial mRNA in trypanosomes.RNA (New York, N.Y.) · 2018Article
- The divergent N-terminal domain of Tim17 is critical for its assembly in the TIM complex in Trypanosoma brucei.Molecular and biochemical parasitology · 2017Article
- U-Insertion/Deletion mRNA-Editing Holoenzyme: Definition in Sight.Trends in parasitology · 2016Review
- Multifunctional G-rich and RRM-containing domains of TbRGG2 perform separate yet essential functions in trypanosome RNA editing.Eukaryotic cell · 2012Article
- Endonuclease associations with three distinct editosomes in Trypanosoma brucei.The Journal of biological chemistry · 2011Article
- TbRGG2 facilitates kinetoplastid RNA editing initiation and progression past intrinsic pause sites.RNA (New York, N.Y.) · 2010Article
- OB-fold domain of KREPA4 mediates high-affinity interaction with guide RNA and possesses annealing activity.RNA (New York, N.Y.) · 2010Article
- The zinc-fingers of KREPA3 are essential for the complete editing of mitochondrial mRNAs in Trypanosoma brucei.PloS one · 2010Article
- REH2 RNA helicase in kinetoplastid mitochondria: ribonucleoprotein complexes and essential motifs for unwinding and guide RNA (gRNA) binding.The Journal of biological chemistry · 2010Article
- Distinct and overlapping functions of MRP1/2 and RBP16 in mitochondrial RNA metabolism.Molecular and cellular biology · 2009Article
- Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome.RNA (New York, N.Y.) · 2009Article
- Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase.RNA (New York, N.Y.) · 2009Article
- The KREPA3 zinc finger motifs and OB-fold domain are essential for RNA editing and survival of Trypanosoma brucei.Molecular and cellular biology · 2008Article
- Trypanosoma brucei ATPase subunit 6 mRNA bound to gA6-14 forms a conserved three-helical structure.RNA (New York, N.Y.) · 2008Article
- TbRGG2, an essential RNA editing accessory factor in two Trypanosoma brucei life cycle stages.The Journal of biological chemistry · 2008Article
- gRNA/pre-mRNA annealing and RNA chaperone activities of RBP16.RNA (New York, N.Y.) · 2008Article
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Authors and funding
4 authors.
Funding
Abstract
RBP16 is an abundant RNA binding protein from Trypanosoma brucei mitochondria that affects both RNA editing and stability. We report here experiments aimed at elucidating the mechanism of RBP16 function in RNA editing. In in vitro RNA editing assays, recombinant RBP16 is able to significantly stimulate insertion editing of both CYb and A6 pre-mRNAs. Enhancement of in vitro editing activity occurs at, or prior to, the step of pre-mRNA cleavage, as evidenced by increased accumulation of pre-mRNA 3' cleavage products in the presence of RBP16. Mutated RBP16 that is severely compromised in cold shock domain (CSD)-mediated RNA binding was able to enhance editing to levels comparable to the wild-type protein in some assays at the highest RBP16 levels tested. However, at low RBP16 concentrations or in assays with native, oligo(U)-tail-bearing gRNAs, editing stimulation by mutant RBP16 was somewhat compromised. Together, these results indicate that both the N-terminal CSD and C-terminal RGG RNA binding domains of RBP16 are required for maximal editing stimulation. Finally, the relaxed specificity of RBP16 for stimulation of both CYb and A6 editing in vitro implicates additional specificity factors that account for the strict CYb specificity of RBP16 action in editing in vivo. Our results constitute the first report of any putative RNA editing accessory factor eliciting an effect on editing in vitro. Overall, these results support a novel accessory role for RBP16 in U insertion editing.
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