ArticleAnalytical chemistry2006
Mass defect labeling of cysteine for improving peptide assignment in shotgun proteomic analyses.
Article in Analytical chemistry, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Investigation and Development of the BODIPY-Embedded Isotopic Signature for Chemoproteomics Labeling and Targeted Profiling.Journal of the American Society for Mass Spectrometry · 2024Article
- Iodine-Containing Mass-Defect-Tuned Dendrimers for Use as Internal Mass Spectrometry Calibrants.Journal of the American Society for Mass Spectrometry · 2018Article
- Mass Defect from Nuclear Physics to Mass Spectral Analysis.Journal of the American Society for Mass Spectrometry · 2017Article
- Development of IsoTaG, a Chemical Glycoproteomics Technique for Profiling Intact N- and O-Glycopeptides from Whole Cell Proteomes.Journal of proteome research · 2017Article
- Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells.Analytical chemistry · 2017Article
- Use of singular value decomposition analysis to differentiate phosphorylated precursors in strong cation exchange fractions.Electrophoresis · 2014Article
- A small azide-modified thiazole-based reporter molecule for fluorescence and mass spectrometric detection.Beilstein journal of organic chemistry · 2014Article
- Use of theoretical peptide distributions in phosphoproteome analysis.Journal of proteome research · 2013Article
- Improved mass defect model for theoretical tryptic peptides.Analytical chemistry · 2012Article
- Identification of Phosphorylated Human Peptides by Accurate Mass Measurement Alone.International journal of mass spectrometry · 2011Article
- Examining troughs in the mass distribution of all theoretically possible tryptic peptides.Journal of proteome research · 2011Article
- Isotopic signature transfer and mass pattern prediction (IsoStamp): an enabling technique for chemically-directed proteomics.ACS chemical biology · 2011Article
- Simultaneous quantitation of amino acid mixtures using clustering agents.Journal of the American Society for Mass Spectrometry · 2011Article
- A simple and robust method for determining the number of basic sites in peptides and proteins using electrospray ionization mass spectrometry.Analytical chemistry · 2011Article
- Oxidative protein labeling in mass-spectrometry-based proteomics.Analytical and bioanalytical chemistry · 2010Review
- Shifting unoccupied spectral space in mass spectrum of peptide fragment ions.Journal of the American Society for Mass Spectrometry · 2009Article
- Direct quantitation of peptide mixtures without standards using clusters formed by electrospray ionization mass spectrometry.Analytical chemistry · 2009Article
- Application of fractional mass for the identification of peptide-oligonucleotide cross-links by mass spectrometry.Journal of mass spectrometry : JMS · 2008Article
- Systematic characterization of high mass accuracy influence on false discovery and probability scoring in peptide mass fingerprinting.Analytical biochemistry · 2008Article
- Accurate mass measurements in proteomics.Chemical reviews · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
A method for improving the identification of peptides in a shotgun proteome analysis using accurate mass measurement has been developed. The improvement is based upon the derivatization of cysteine residues with a novel reagent, 2,4-dibromo-(2'-iodo)acetanilide. The derivitization changes the mass defect of cysteine-containing proteolytic peptides in a manner that increases their identification specificity. Peptide masses were measured using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron mass spectrometry. Reactions with protein standards show that the derivatization of cysteine is rapid and quantitative, and the data suggest that the derivatized peptides are more easily ionized or detected than unlabeled cysteine-containing peptides. The reagent was tested on a 15N-metabolically labeled proteome from M. maripaludis. Proteins were identified by their accurate mass values and from their nitrogen stoichiometry. A total of 47% of the labeled peptides are identified versus 27% for the unlabeled peptides. This procedure permits the identification of proteins from the M. maripaludis proteome that are not usually observed by the standard protocol and shows that better protein coverage is obtained with this methodology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.