ArticleWorld journal of gastroenterology2006
Helicobacter infection in hepatocellular carcinoma tissue.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 64 citations in OpenAlex.
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- Cancer risk in chronic rhinosinusitis: a propensity score matched case-control cohort study.American journal of translational research · 2019Article
- Helicobacter infection in the hepatobiliary system and hepatic lesions: a possible association in dogs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
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- Helicobacter pylori Infection as a Risk Factor for Hepatocellular Carcinoma: A Case-Control Study in Ethiopia.International journal of hepatology · 2018Article
- Long-term risk of gastrointestinal cancers in persons with gastric or duodenal ulcers.Cancer medicine · 2016Article
- Extragastric manifestations of Helicobacter pylori infection: Possible role of bacterium in liver and pancreas diseases.World journal of hepatology · 2015Review
- From the stomach to other organs: Helicobacter pylori and the liver.World journal of hepatology · 2015Review
- Association between Helicobacter spp. infections and hepatobiliary malignancies: a review.World journal of gastroenterology · 2015Review
- Article
- Novel aspects of the liver microenvironment in hepatocellular carcinoma pathogenesis and development.International journal of molecular sciences · 2014Review
- Effects of Helicobacter pylori γ-glutamyltranspeptidase on apoptosis and inflammation in human biliary cells.Digestive diseases and sciences · 2012Article
- Helicobacter pylori in Thai patients with cholangiocarcinoma and its association with biliary inflammation and proliferation.HPB : the official journal of the International Hepato Pancreato Biliary Association · 2012Article
- Helicobacter Pylori DNA in Liver Tissues From Chronic Hepatitis C Egyptian Patients.Gastroenterology research · 2011Article
- Contribution of gut bacteria to liver pathobiology.Gastroenterology research and practice · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo investigate whether Helicobacter species (Helicobacter spp.) could be detected in hepatocellular carcinoma (HCC) tissue.
methodsLiver samples from 28 patients with hepatocellular carcinoma (HCC) diagnosed by histopathology were studied. Twenty-two patients with other liver diseases (5 with liver trauma, 7 with cavernous liver hemangioma, 6 with liver cyst and 4 with hepatolithiasis), 25 patients with gastric cancer, 15 with colonic cancer and 15 with myoma of uterus served as controls. Two pieces of biopsy were obtained from each patient. One was cultured for Helicobacter spp. and extraction of DNA, the other was prepared for scanning electron microscopy (SEM) and in situ hybridization. The samples were cultured on Columbia agar plates with microaerobic techniques. Helicobacter spp. in biopsy from the studied subjects was detected by polymerase chain reaction (PCR) with Helicobacter spp. 16S rRNA primers. Amplified products were identified by Southern hybridization and sequenced further. Besides, other genes (vacA, cagA) specific for Helicobacter pylori (H pylori) were also detected by PCR. Helicobacter spp. in biopsies was observed by SEM. Transmission electron microscopy (TEM) was performed to identify the cultured positive Helicobacter spp. The presence of Helicobacter spp. was detected by in situ hybridization to confirm the type of Helicobacter.
resultsThe positive rate of Helicobacter cultured in HCC and gastric cancer tissue was 10.7% (3/28) and 24% (6/25), respectively. Helicobacter microorganisms were identified further by typical appearance on Gram staining, positive urease test and characteristic colony morphology on TEM. The bacterium was observed in adjacent hepatocytes of the two HCC samples by SEM. The number of cocci was greater than that of bacilli. The bacterium was also found in four gastric cancer samples. PCR showed that the positive rate of HCC and gastric cancer samples was 60.7% and 72% respectively, while the controls were negative (P<0.01). The PCR-amplified products were identified by Southern hybridization and sequenced. The homology to 16S rRNA of H pylori was 97.80%. The samples were verified by in situ hybridization for Helicobacter spp. 16S rRNA-mRNA and proved to be H pylori positive. There was no statistical significance between HCC and gastric cancer (P>0.05), but the positive rate of HCC and controls had statistical significance (P<0.01). Only 3 HCC samples and 2 gastric cancer samples of the cagA genes were detected. None of the samples reacted with primers for vacA in the two groups. As for the genotype of H pylori, type II had preference over type I.
conclusionHelicobacter infection exists in liver tissues of HCC patients. Helicobacter spp. infection is related with HCC, which needs further research.
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