ArticleWorld journal of gastroenterology2006
Anticancer and cytotoxic properties of the latex of Calotropis procera in a transgenic mouse model of hepatocellular carcinoma.
Article in World journal of gastroenterology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 142 citations in OpenAlex.
- Novel cyclic CCancer cell international · 2025Article
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- Potential anticancer properties of calotropis procera: An investigation on breast and colon cancer cells.Heliyon · 2023Article
- In Vitro: The Effects of the Anticoccidial Activities ofMolecules (Basel, Switzerland) · 2023Article
- The Activity ofInternational journal of molecular sciences · 2022Article
- A Review of Medicinal Plants of the Himalayas with Anti-Proliferative Activity for the Treatment of Various Cancers.Cancers · 2022Review
- Anticancer, Anticoagulant, Antioxidant and Antimicrobial Activities ofMolecules (Basel, Switzerland) · 2022Article
- Tap the sap - investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals.Frontiers in plant science · 2022Review
- Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids.International journal of molecular sciences · 2021Review
- Neuropharmacological Assessment of the Hydroethanolic Leaf Extract ofScientifica · 2021Article
- BclVeterinary research forum : an international quarterly journal · 2021Article
- Awareness and current knowledge of epilepsy.Metabolic brain disease · 2020Review
- Effects of Plant and Animal Natural Products on Mitophagy.Oxidative medicine and cellular longevity · 2020Review
- Cytotoxicity against tumor cell lines and anti-inflammatory properties of chitinases from Calotropis procera latex.Naunyn-Schmiedeberg's archives of pharmacology · 2017Article
- Transcriptome and Metabolite analysis reveal candidate genes of the cardiac glycoside biosynthetic pathway from Calotropis procera.Scientific reports · 2016Article
- Strophalloside induces apoptosis of SGC-7901 cells through the mitochondrion-dependent caspase-3 pathway.Molecules (Basel, Switzerland) · 2015Article
- Antiedematogenic and antioxidant properties of high molecular weight protein sub-fraction of Calotropis procera latex in rat.Journal of basic and clinical pharmacy · 2015Article
- Cysteine Protease Profiles of the Medicinal Plant Calotropis procera R. Br. revealed by de novo transcriptome analysis.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the anticancer property of the dried latex (DL) of Calotropis procera, a tropical medicinal plant, in the X15-myc transgenic mouse model of hepatocellular carcinoma and to elucidate its mechanism of action in cell culture.
methodsThe young transgenic mice were orally fed with the aqueous suspension of DL (400 mg/kg for 5 d/wk) for 15 wk and their liver was examined for histopathological changes at 20 wk. Serum levels of vascular endothelial growth factor (VEGF) were also measured in these animals. To characterize the active fraction, DL was extracted with petroleum ether followed by methanol. The methanolic extract was sub-fractionated on a silica gel G column using a combination of non-polar and polar solvents and eleven fractions were obtained. Each fraction was analysed for cytotoxic effect on hepatoma (Huh7) and non-hepatoma (COS-1) cell lines and non-transformed hepatocytes (AML12) using tetrazolium (MTT) assay. Finally, the mechanism of cell death was investigated by measuring the levels of Bcl2, caspase 3 and DNA fragmentation.
resultsDL treatment of mice showed a complete protection against hepatocarcinogenesis. No adverse effect was observed in these animals. The serum VEGF level was significantly lowered in the treated mice as compared to control animals. Cell culture studies revealed that the methanolic extract of DL as well as its fraction 8 induced extensive cell death in both Huh-7 and COS-1 cells while AML12 cells were spared. This was accompanied by extensive fragmentation of DNA in Huh-7 and COS-1 cells. No change in the levels of canonical markers of apoptosis such as Bcl2 and caspase 3 was observed.
conclusionDL of C. procera has the potential for anti-cancer therapy due to its differentiable targets and non-interference with regular pathway of apoptosis.
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