ArticleThe American journal of tropical medicine and hygiene2006
A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes.
Article in The American journal of tropical medicine and hygiene, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
78 citing papers in PubMed, 157 citations in OpenAlex.
- Integrating Community Practices, Vector Resistance, and Molecular Xenomonitoring of Wuchereria Bancrofti: A One Health Implementation Study in Lymphatic Filariasis-Endemic Communities.Acta parasitologica · 2026Article
- Circulating Cell-Free Nucleic Acid Detection for Wuchereria bancrofti Infections.The Journal of infectious diseases · 2025Article
- Assessing the need to implement mass drug administration against Wuchereria bancrofti infection using both human serology and xenomonitoring in the urban conurbation of Monrovia, Liberia.PLoS neglected tropical diseases · 2025Article
- Article
- Current perspectives in the epidemiology and control of lymphatic filariasis.Clinical microbiology reviews · 2025Review
- Evaluation of sampling strategies for assessing lymphatic filariasis endemic status of a non-MDA district in South India.PLoS neglected tropical diseases · 2025Article
- Wolbachia bacteria in Mansonella perstans isolates from patients infected in different geographical areas: a pilot study from the ESCMID Study Group for Clinical Parasitology.Parasites & vectors · 2025Article
- Cell-free DNA as a complementary diagnostic tool for neglected tropical diseases towards achieving the WHO NTDs elimination by 2030.The journal of liquid biopsy · 2025Article
- The role of molecular xenomonitoring for assessing the impact of Ivermectin, Diethylcarbamazine Citrate and Albendazole triple-drug treatment against lymphatic filariasis: A cross-sectional study in the Kenyan coastal region.PLOS global public health · 2025Article
- Review
- Detection of Wuchereria bancrofti DNA in wild caught vector and non-vector mosquitoes: implications for elimination of lymphatic filariasis.Molecular biology reports · 2024Article
- Wuchereria bancrofti infection is associated with progression to clinical visceral leishmaniasis in VL- endemic areas in Muzaffarpur, Bihar, India.PLoS neglected tropical diseases · 2023Article
- Longitudinal study of cross-reactive antigenemia in individuals with highFrontiers in parasitology · 2023Article
- No evidence of lymphatic filariasis transmission in Bamako urban setting after three mass drug administration rounds.Parasitology research · 2022Article
- Real Time PCR-based diagnosis of human visceral leishmaniasis using urine samples.PLOS global public health · 2022Article
- Article
- Development and Validation of Novel PCR Assays for the Diagnosis of Bovine Stephanofilariasis and Detection ofPathogens (Basel, Switzerland) · 2021Article
- Multi-centric evaluation of a stage-specific reverse transcriptase-polymerase chain reaction assay as a xenomonitoring tool for the detection of infective (LThe Indian journal of medical research · 2021Article
- Clinical, serological and DNA testing in Bengo Province, Angola further reveals low filarial endemicity and opportunities for disease elimination.Parasite epidemiology and control · 2020Article
- Molecular xenomonitoring of diurnally subperiodic Wuchereria bancrofti infection in Aedes (Downsiomyia) niveus (Ludlow, 1903) after nine rounds of Mass Drug Administration in Nancowry Islands, Andaman and Nicobar Islands, India.PLoS neglected tropical diseases · 2020Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
We developed and evaluated real-time polymerase chain reaction (PCR) assays for detecting Wuchereria bancrofti DNA in human blood and in mosquitoes. An assay based on detection of the W. bancrofti "LDR" repeat DNA sequence was more sensitive than an assay for Wolbachia 16S rDNA. The LDR-based assay was sensitive for detecting microfilarial DNA on dried membrane filters or on filter paper. We also compared real-time PCR with conventional PCR (C-PCR) for detecting W. bancrofti DNA in mosquito samples collected in endemic areas in Egypt and Papua New Guinea. Although the two methods had comparable sensitivity for detecting filarial DNA in reference samples, real-time PCR was more sensitive than C-PCR in practice with field samples. Other advantages of real-time PCR include its high-throughput capacity and decreased risk of cross-contamination between test samples. We believe that real-time PCR has great potential as a tool for monitoring progress in large-scale filariasis elimination programs.
Indexed as
Identifiers
16687688PMC2196401W2124884903What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.