ArticleBiology of reproduction2006
Poor centrosomal function of cat testicular spermatozoa impairs embryo development in vitro after intracytoplasmic sperm injection.
Article in Biology of reproduction, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Embryonic mosaicism in severe male factor infertility is unrelated to sperm retrieval technique.Journal of assisted reproduction and genetics · 2026Article
- A 30-year journey in wildlife assisted reproductive technologies.Animal reproduction · 2026Review
- Abnormal centriolar biomarker ratios correlate with unexplained bull artificial insemination subfertility: a pilot study.Scientific reports · 2023Article
- Transfer of Galectin-3-Binding Protein via Epididymal Extracellular Vesicles Promotes Sperm Fertilizing Ability and Developmental Potential in the Domestic Cat Model.International journal of molecular sciences · 2023Article
- RNA-sequencing attest increased sperm motility in bovine spermatozoa treated with ethanolic extract of3 Biotech · 2023Article
- Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.Journal of assisted reproduction and genetics · 2022Article
- Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model.Journal of assisted reproduction and genetics · 2021Article
- Time-lapse imaging of human embryos fertilized with testicular sperm reveals an impact on the first embryonic cell cycle.Biology of reproduction · 2021Article
- Comparison of the Morphology and Developmental Potential of Oocytes Obtained from Prepubertal and Adult Domestic and Wild Cats.Animals : an open access journal from MDPI · 2020Article
- Novel Proteomic Profiling of Epididymal Extracellular Vesicles in the Domestic Cat Reveals Proteins Related to Sequential Sperm Maturation with Differences Observed between Normospermic and Teratospermic Individuals.Molecular & cellular proteomics : MCP · 2020Article
- High rates of aneuploidy, mosaicism and abnormal morphokinetic development in cases with low sperm concentration.Journal of assisted reproduction and genetics · 2020Article
- Development of feline embryos produced by Piezo-actuated intracytoplasmic sperm injection of elongated spermatids.The Journal of reproduction and development · 2019Article
- It takes two (centrioles) to tango.Reproduction (Cambridge, England) · 2019Review
- The Role of Sperm Centrioles in Human Reproduction - The Known and the Unknown.Frontiers in cell and developmental biology · 2019Review
- Azoospermia and embryo morphokinetics: testicular sperm-derived embryos exhibit delays in early cell cycle events and increased arrest prior to compaction.Journal of assisted reproduction and genetics · 2018Article
- The mutual benefits of research in wild animal species and human-assisted reproduction.Journal of assisted reproduction and genetics · 2018Review
- Key factors enhancing sperm fertilizing ability are transferred from the epididymis to the spermatozoa via epididymosomes in the domestic cat model.Journal of assisted reproduction and genetics · 2018Article
- Dry Preservation of Spermatozoa: Considerations for Different Species.Biopreservation and biobanking · 2017Review
- Occurrence and functional significance of the transcriptome in bovine (Bos taurus) spermatozoa.Scientific reports · 2017Article
- Asterless Reduction during Spermiogenesis Is Regulated by Plk4 and Is Essential for Zygote Development in Drosophila.Current biology : CB · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In the domestic cat, morula-blastocyst formation in vitro is compromised after intracytoplasmic sperm injection (ICSI) with testicular compared to ejaculated spermatozoa. The aim of this study was to determine the cellular basis of the lower developmental potential of testicular spermatozoa. Specifically, we examined the influence of sperm DNA fragmentation (evaluated by TUNEL assay) and centrosomal function (assessed by sperm aster formation after ICSI) on first-cleavage timing, developmental rate, and morula-blastocyst formation. Because the incidences of DNA fragmentation were not different between testicular and ejaculated sperm suspensions, DNA integrity was not the origin of the reduced developmental potential of testicular spermatozoa. After ICSI, proportions of fertilized and cleaved oocytes were similar and not influenced by sperm source. However, observations made at 5 h postactivation clearly demonstrated that 1) zygotes generally contained a large sperm aster after ICSI with ejaculated spermatozoa, a phenomenon never observed with testicular spermatozoa, and 2) proportions of zygotes with short or absent sperm asters were higher after ICSI with testicular spermatozoa than using ejaculated spermatozoa. The poor pattern of aster formation arose from the testicular sperm centrosome, which contributed to a delayed first cleavage, a slower developmental rate, and a reduced formation of morulae and blastocysts compared to ejaculated spermatozoa. When a testicular sperm centrosome was replaced by a centrosome from an ejaculated spermatozoon, kinetics of first cell cycle as well as embryo development quality significantly improved and were comparable to data from ejaculated spermatozoa. Results demonstrate for the first time in mammals that maturity of the cat sperm centrosome (likely via epididymal transit) contributes to an enhanced ability of the spermatozoon to produce embryos that develop normally to the morula and blastocyst stages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.