ArticleJournal of lipid research2006
Characterization and direct quantitation of sphingoid base-1-phosphates from lipid extracts: a shotgun lipidomics approach.
Article in Journal of lipid research, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
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- A Sphingolipidomic Profiling Approach for Comparing X-ray-Exposed and Unexposed HepG2 Cells.International journal of molecular sciences · 2023Article
- Acid sphingomyelinase/ceramide system in schizophrenia: implications for therapeutic intervention as a potential novel target.Translational psychiatry · 2022Review
- Lithium Hydroxide Hydrolysis Combined with MALDI TOF Mass Spectrometry for Rapid Sphingolipid Detection.Journal of the American Society for Mass Spectrometry · 2021Article
- Sphingolipid metabolism as a marker of hepatotoxicity in drug-induced liver injury.Prostaglandins & other lipid mediators · 2020Article
- O-cyclic phytosphingosine-1-phosphate stimulates HIF1α-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells.Cell death & disease · 2019Article
- Shotgun Sphingolipid Analysis of Human Aqueous Humor.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why?Mass spectrometry reviews · 2017Review
- Improved Butanol-Methanol (BUME) Method by Replacing Acetic Acid for Lipid Extraction of Biological Samples.Lipids · 2016Article
- Advanced Shotgun Lipidomics for Characterization of Altered Lipid Patterns in Neurodegenerative Diseases and Brain Injury.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Applications of mass spectrometry for cellular lipid analysis.Molecular bioSystems · 2015Review
- S1P control of endothelial integrity.Current topics in microbiology and immunology · 2014Review
- Multidimensional mass spectrometry-based shotgun lipidomics.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Sphingosine-1-phosphate lyase expression in embryonic and adult murine tissues.Journal of lipid research · 2012Article
- Lipidomics reveals a remarkable diversity of lipids in human plasma.Journal of lipid research · 2010Article
- Automated lipid identification and quantification by multidimensional mass spectrometry-based shotgun lipidomics.Analytical chemistry · 2009Article
- Direct quantitation of psychosine from alkaline-treated lipid extracts with a semi-synthetic internal standard.Journal of lipid research · 2009Article
- Subtype-specific residues involved in ligand activation of the endothelial differentiation gene family lysophosphatidic acid receptors.The Journal of biological chemistry · 2008Article
- Alkaline methanolysis of lipid extracts extends shotgun lipidomics analyses to the low-abundance regime of cellular sphingolipids.Analytical biochemistry · 2007Article
- Potential mechanisms contributing to sulfatide depletion at the earliest clinically recognizable stage of Alzheimer's disease: a tale of shotgun lipidomics.Journal of neurochemistry · 2007Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here, we have extended shotgun lipidomics for the characterization and quantitation of sphingosine-1-phosphate (S1P) and dihydrosphingosine-1-phosphate (DHS1P) in crude lipid extracts in the presence of ammonium hydroxide by using precursor ion scanning of m/z 79.0 (corresponding to [PO(3)](-)) in the negative-ion mode. It is demonstrated that a broad linear dynamic range for the quantitation of both S1P and DHS1P and a detection limit at low amol/mul concentration are achieved using this approach. The developed method for the quantitation of sphingoid base-1-phosphates is generally simpler and more efficient than other previously published methods. Multiple factors influencing the quantitation of sphingoid base-1-phosphates, including ion suppression, extraction efficiency, and potential overlapping with other molecular species, were examined extensively and/or are discussed. Mass levels of S1P and DHS1P in multiple biological samples, including human plasma, mouse plasma, and mouse brain tissues (e.g., cortex, cerebellum, spinal cord, and brain stem), were determined by the developed methodology. Accordingly, this technique, as a new addition to shotgun lipidomics technology, will be extremely useful for understanding the pathways of sphingolipid metabolism and for exploring the important roles of sphingoid base-1-phosphates in a wide range of physiological and pathological studies.
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