ArticleCell biochemistry and biophysics2006
Single-step multicolor fluorescence in situ hybridization using semiconductor quantum dot-DNA conjugates.
Article in Cell biochemistry and biophysics, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- Dextran-Assisted Biomimetic Fabrication of CdSe:Eu QDs With Enhanced Biocompatibility and Stability.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets.Beilstein journal of nanotechnology · 2017Article
- Quantum dots-DNA bioconjugates: synthesis to applications.Interface focus · 2016Review
- Assessing the stochastic intermittency of single quantum dot luminescence for robust quantification of biomolecules.Analytical chemistry · 2013Article
- Nanotechnology and molecular cytogenetics: the future has not yet arrived.Nano reviews · 2010Article
- Lighting up individual DNA binding proteins with quantum dots.Nano letters · 2009Article
- Suppression of quantum dot blinking in DTT-doped polymer films.The journal of physical chemistry. C, Nanomaterials and interfaces · 2009Article
- Quantum dots as new-generation fluorochromes for FISH: an appraisal.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Luminescent lanthanide nanoparticles as labels in DNA microarrays for quantification of methyl tertiary butyl ether degrading bacteria.Journal of nanoscience and nanotechnology · 2008Article
- Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates.Chromosoma · 2008Article
- Potential clinical applications of quantum dots.International journal of nanomedicine · 2008Review
- Semiautomated multiplexed quantum dot-based in situ hybridization and spectral deconvolution.The Journal of molecular diagnostics : JMD · 2007Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
We report a rapid method for the direct multicolor imaging of multiple subnuclear genetic sequences using novel quantum dot-based fluorescence in situ hybridization (FISH) probes (QD-FISH). Short DNA oligonucleotides were attached on QDs and used in a single hybridization/detection step of target sites in situ. QD-FISH probes penetrate both intact interphase nuclei and metaphase chromosomes and showed good targeting of dense chromatin domains with minimal steric hindrances. We further demonstrated that QD's broad absorption spectra allowed different colored probes specific for distinct subnuclear genetic sequences to be simultaneously excited with a single excitation wavelength and imaged free of chromatic aberrations in a single exposure. Thus, these results demonstrate that QD-FISH probes are very effective in multicolor FISH applications. This work also documents new possibilities of using QD-FISH probes detection down to the single molecule level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.