ArticleThe Plant cell2006
The Galpha protein controls a pH-dependent signal path to the induction of phytoalexin biosynthesis in Eschscholzia californica.
Article in The Plant cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 48 citations in OpenAlex.
- The Arabidopsis thaliana non-specific phospholipase C2 is involved in the response to Pseudomonas syringae attack.Annals of botany · 2018Article
- The Vacuolar Proton-Cation Exchanger EcNHX1 Generates pH Signals for the Expression of Secondary Metabolism in Eschscholzia californica.Plant physiology · 2016Article
- "Self" and "non-self" in the control of phytoalexin biosynthesis: plant phospholipases A2 with alkaloid-specific molecular fingerprints.The Plant cell · 2015Article
- How plants cope with heavy metals.Botanical studies · 2014Review
- The salt stress-induced LPA response in Chlamydomonas is produced via PLA₂ hydrolysis of DGK-generated phosphatidic acid.Journal of lipid research · 2011Article
- Phospholipases in action during plant defense signaling.Plant signaling & behavior · 2011Review
- Structure and mechanism of sanguinarine reductase, an enzyme of alkaloid detoxification.The Journal of biological chemistry · 2010Article
- Primary metabolism of chickpea is the initial target of wound inducing early sensed Fusarium oxysporum f. sp. ciceri race I.PloS one · 2010Article
- Plant phospholipid signaling: "in a nutshell".Journal of lipid research · 2009Article
- The signal molecule lysophosphatidylcholine in Eschscholzia californica is rapidly metabolized by reacylation.Planta · 2008Article
- Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1.Plant cell reports · 2007Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The function of a Galpha protein in the elicitation of phytoalexin (benzophenanthridine) biosynthesis was characterized in cultured cells of California poppy (Eschscholzia californica). Both the decrease of Galpha content via antisense transformation and the expression of recombinant anti-Galpha single-chain antibodies strongly impaired the induction of alkaloid biosynthesis by low elicitor concentrations. All transgenic cell types were deficient in two elicitor-triggered early signal events: activation of phospholipase A2 (PLA2) and efflux of vacuolar protons. The lacking H+ efflux could be restored (1) by adding lysophosphatidylcholine (LPC), a product of PLA2 activity, to vacuoles in situ and (2) by exposing intact cells to isotonic, near-neutral HEPES buffers. The latter treatment induced alkaloid biosynthesis in the absence of elicitor and in Galpha-deficient cells. We conclude that Galpha mediates the stimulation of PLA2 by low elicitor concentrations and that the resulting peak of LPC initiates a transient efflux of vacuolar protons. In this way, an acidic peak of the cytoplasmic pH is generated that causes the expression of enzymes of phytoalexin production independent of the hypersensitive response.
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